Sep 3, 2010 · We found that PCBP4 expression decreases cyclin-dependent kinase inhibitor p21 induction in response to DNA damage. We also provided evidence that PCBP4 regulates p21 expression independently of p53. In addition, we showed that a deficiency in PCBP4 enhances p21 induction upon DNA damage.

Here, we find that RNase L regulates p21-mediated G 1 growth arrest in AU-rich elements-dependent manner. We found a significant loss of p21 mRNA expression in RNASEL−/− MEFs and that the overexpression of RNase L in HeLa cells induces p21 mRNA expression.

To verify the microarray results, we performed RT–PCR analysis on specific genes in the pathways identified by the data analyses. Knockdown of p21 led to reduced expression of collagen, type I, alpha 1 (COL1A1) and increased the mRNA levels of the cell cycle regulators CDK‐1 and cyclin A2 (Fig 2F).

Oct 7, 2013 · Here, we demonstrate that the cyclin-dependent kinase inhibitor p21 is both necessary and sufficient for the downregulation of known p53-repression targets, including survivin, CDC25C, and CDC25B...

To determine the mechanism of the FGF2-boosted proliferation of aged satellite cells, we examined the expression of the CDKIs p16 and p21 using real-time q-RT-PCR and Western Blotting.

Sep 14, 2017 · We observed extra-telomeric binding of the telomere repeat binding factor TRF2 within the promoter of the cyclin-dependent kinase CDKNIA (p21/CIP1/WAF1). This result in TRF2 induced transcription...

RT-PCR analysis confirmed that Tax1 significantly upregulated p21 in CD34 ϩ /CD38 Ϫ and CD34 ϩ /CD38 ϩ subpopulations (5-fold and 13.5-fold, respectively) (Fig. 4A).

In the present study the kinetics of the changes of the P21 transcription in Caco-2 colon adenocarcinoma cells treated with various concentrations of sodium butyrate was determined using a novel real-time quantitative RT-PCR (TaqMan) technique.

Apr 11, 2017 · Three human colorectal cancer cell lines, HCT-116, HCT-116 (p53-/-) and HT-29 were transfected with the p21-saRNA-322. The expression of P21 protein and p21 mRNA were measured using the Western blot and reverse transcriptase polymerase chain reaction (RT-PCR).

Jun 20, 2016 · We report that a subset of atypical cancerous cells strongly expressing p21 showed proliferation features. This occurred predominantly in p53-mutant human cancers, suggesting p53-independent...