Aug 28, 2017 · We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and...

We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content.

Oct 26, 2020 · Here we describe an updated and optimized protocol for ATAC-seq, called Omni-ATAC, that is applicable across a broad range of cell and tissue types. The ATAC-seq workflow has five main...

Reportedly, this revised protocol (Omni-ATAC) can be performed on snap-frozen cells or tissue [3]. Alternatively, you can slowly freeze cells using the protocol detailed in [4] (resuspend pellet in CryoStor buffer 50 ul / 50,000 cells and freeze at -80oC in Mr. Frosty device).

detailed explanation of how to properly amplify ATAC-seq libraries. Using the Omni-ATAC protocol, we find that the number of cycles of amplification required is very low.

3 days ago · This protocol describes methods for performing ATACseq.

We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works universally across multiple cell types and cell contexts with significant improvement of signal-to-background ratio and information content.

To optimize the ATAC-seq method to frozen adipose tissue and gain high data reproducibility in various tissues, we modified the Omni-ATAC protocol to include an effective bead-based tissue homogenization process that is applicable to adipose tissue and skeletal muscle.

Omni-ATAC: an improved ATAC-seq protocol for chromatin accessibility profiling in cells or tissues with reduced mitochondria DNA contamination and substantial improvement of signal-to-background ratio.

Omni-ATAC is an improved ATAC-seq protocol for chromatin accessibility profiling. The Omni-ATAC protocol lowers sequencing costs by generating fewer sequencing reads that map to mitochondrial DNA, consequently a higher percentage of …