May 16, 2023 · Seahorse能量代谢分析仪 是一种用于测量细胞能量代谢的仪器，可以通过测量细胞的耗氧率（OCR）和胞外酸化率（ECAR）来评估细胞的线粒体功能、 糖酵解 和氧化磷酸化等过程。 OCR和ECAR的测量原理如下（图1.1）： OCR（Oxygen Consumption Rate）： OCR是指细胞对氧气的消耗速率。 Seahorse能量代谢分析仪通过测量细胞周围培养基中的溶解氧含量的变化来确定OCR。 在测量过程中，细胞被置于特定的耗氧试剂中，如噻唑类化合物（例如， …

We detail a protocol for measurement of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) measurements in living cells, utilizing the Seahorse XF24 Extracellular Flux Analyzer (Figure 1).

Agilent Seahorse XF Analyzers perform automatic, real-time measurements of oxygen consumption rate (OCR), proton efflux rate (PER) or extracellular acidification rate (ECAR), as well as ATP production rates of live cells in a multi-well plate, interrogating key cellular functions like mitochondrial respiration and glycolysis.

Jan 11, 2021 · There are two common methods currently employed to measure OCR: 1) a potentiometric method that uses a platinum hydrogen (Clark) electrode, and 2) quenching of the fluorescence lifetime of metal-porphyrin complexes (MPCs) …

Specifically, the extracellular acidification rate (ECAR), which approximates glycolytic activity under certain conditions, and the mitochondrial oxygen consumption rate (OCR), which is a key metric of mitochondrial function, can be determined simultaneously within the same small population of hPSCs using a commercially available XF24 ...

May 10, 2012 · Specifically, the extracellular acidification rate (ECAR), which approximates glycolytic activity under certain conditions, and the mitochondrial oxygen consumption rate (OCR), which is a...

Specifically, this machine measures glycolysis by analyzing the extracellular acidification rate (ECAR) and measures mitochondrial oxidative phosphorylation on the basis of the oxygen consumption rate (OCR), through real-time and live cell analysis.

It allows simultaneous real-time measurements of both OCR and extracellular acidification rate (ECAR) in multiple cell lines and conditions, reducing the amount of required sample material and increasing the throughput [18, 19].

OCR: oxygen consumption rate, a direct measure of mito-chondrial respiration. When measured under normal and stressed conditions, it can reveal defects in mitochondrial function and/or respiratory capacity. ECAR: extracellular acidification associated with lactate efflux; increases indicate cellular activation and prolifera-tion.

We detail a protocol for measurement of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) measurements in living cells, utilizing the Seahorse XF24 Extracellular Flux Analyzer (Figure 1).