The NNN (Novy-MacNeal-Nicolle) is a common culture medium for isolating the agent of the genus Leishmania, which causes leishmaniasis. The culture of this organism in suitable media gives rise to a motile, extracellular form called promastigote.

Novy–MacNeal–Nicolle medium (NNN) in microbiology is a culture medium used to grow Leishmania – needed when the amastigotes are not found in sufficient quantities by scraping the growth substrate.

The culture of aspirates of bone marrow or spleen or liver biopsies can be useful if the Novy-MacNeal-Nicolle (NNN) medium or other suitable media are available.

This method provides an alternative to tube- or plate-based culture assay using NNN media and blood agar slopes (Boggild et al., 2010; Sacks & Melby, 2015) through the use of a plated culture system and a new combination of antibiotics to prevent the growth of bacteria present on the skin and lesion. For murine experiments the use of gentamicin ...

NNN medium stands for Novy-MacNeal-Nicolle medium and it was developed by Novy, McNeal, and modified by Nicolle. It uses to grow parasites, Leishmania and Trypanosoma. Culture is sensitive than smear and thus used to grow– needed when the amastigotes are not found in sufficient quantities in smear. Ingredients Gms / 100 ml. Part- A.

Mar 21, 2019 · Traditional culture method was performed by using Novy-MacNeal-Nicolle (NNN) medium and Locke’s solution as an overlay media.

Retrospective evaluation of the contribution of Leishmania cultures on NNN medium in leishmaniasis diagnosis have been done using the data of 375 cultures concerning 214 CL cases and 125 VL cases consecutively recruited in Pasteur Institute of Tunisia between 1995 and 2007.

Feb 1, 2009 · We have demonstrated that modified NNN medium is a more sensitive and time-efficient means of isolating Leishmania parasites from cutaneous lesions than traditional culture.

Jun 15, 2018 · The most commonly used culture medium for the in vitro isolation of Leishmania spp. from canine biological samples is biphasic Novy-MacNeal-Nicolle (NNN) medium, whose solid phase is prepared using rabbit blood.

Jul 12, 2017 · The authors have compared the classic culture media to the new media RPMI-PY and Tobie-PY which have been confirmed as achieving a better performance in terms of time and parasitic load with ...