Dec 2, 2016 · One of the major transcriptional regulators in lymphoid cells is NFAT (Nuclear Factor of Activated T Cells), as highlighted by its important role in T and B cell development and function. With regard to NK cells, available data indicate that …

Nov 2, 2023 · In vitro knockout (KO) of NFAT1 (NFATc2) or NFAT2 (NFATc1) enhanced NK cell degranulation and resulted in increased production of granzyme B and perforin upon stimulation of activating receptors like NK1.1 or Nkp46 or upon co …

Sep 14, 2023 · Here we found that NFAT5, an NFAT family transcription factor that lacks an AP-1 docking site, was highly expressed in exhausted CD8 + T cells in the context of chronic infections and tumors but...

Oct 30, 2023 · Generation of CRISPR/Cas9-based knockout (KO) cell line. Guide RNA (gRNA) vectors targeting NFAT1, p65, and cRel were produced, starting with an empty expression vector. The vector was cleaved using BsaI New England (Ipswich, MA, USA) and ligated to each targeted annealed oligonucleotide.

Feb 12, 2024 · To understand how NFAT signaling regulates CD8 + T cell differentiation, we performed detailed transcriptional profiling of NFATc1 KO, NFATc2 KO, NFAT DKO and WT OTI T cells isolated from spleen of acutely infected animals.

Dec 2, 2016 · One of the major transcriptional regulators in lymphoid cells is NFAT (Nuclear Factor of Activated T Cells), as highlighted by its important role in T and B cell development and function. With regard to NK cells, available data indicate that …

Nov 24, 2022 · Adaptation to hypertonic environment is mediated by the actions of the nuclear factor of activated T-cells 5 (NFAT5). NFAT5 induces the expression of genes that lead to intracellular accumulation...

May 4, 2012 · In this study, we investigated the functional importance of the NFAT1 and NF-κB (p65) in IL-9 gene transcription in CD4 + T cells. In vivo binding of NFAT1 and NF-κB (p65) to the IL-9 promoter was observed.

Jun 15, 2022 · NK cells are lymphoid components of innate immunity and play an important role in tumor immunosurveillance. One of the major transcriptional regulators in lymphoid cells is NFAT (Nuclear Factor of Activated T cells), controlling lymphocyte development and activity.

Nov 16, 2012 · At approximately 26 weeks of age, NFAT2 ko mice showed an approximately 40 fold increased lymphocyte count in the peripheral blood than their litter mate controls (1500/μL vs. 60000/μL). Splenomegaly and lymphatic adenopathy was also significantly increased in …