Recombinant Jurkat T-cells expressing an enhanced GFP (eGFP) gene under the control of NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway can be monitored by examining eGFP expression.

Jul 15, 2000 · We have engineered a self-inactivating retroviral vector containing multiple (3 or 6) nuclear factor of activated T-cell (NFAT)-binding sites, followed by the minimal IL2 promoter and the reporter gene GFP. Jurkat cells, primary T-cell blasts, and T-cell clones were transduced with high efficiency (20%-40%).

Jun 1, 2005 · In this Review, I provide an overview of recent advances in our understanding of the regulation, mechanism of action and functions of NFAT proteins in cells of the immune system, with a focus on...

In this study, we generated a novel platform cell line, CD4-2D3, in which GFP reporter was expressed by NFAT activation via TCR signaling, for correctly evaluating functional avidity of HLA class II-restricted TCRs.

Jan 28, 2019 · To detect the signal induced by SABRs, we used NFAT-GFP-Jurkat cells, which express GFP after receiving a signal via CD3ζ.

Jul 15, 2000 · We have engineered a self-inactivating retroviral vector containing multiple (3 or 6) nuclear factor of activated T-cell (NFAT)-binding sites, followed by the minimal IL2 promoter and the reporter gene GFP.

Feb 21, 2013 · Interactions of NFAT-GFP (green) and H2B-mRFP (red)-expressing HA-T CM with HA-peptide pulsed B cells (blue) and unpulsed B cells (white). Some productive interactions inducing NFAT activation and nuclear accumulation, resulting in green-red overlap, are highlighted through circles.

In the 2D3, the precise TCR functional avidity of transduced TCRs is easily evaluable by the expression of green fluorescent protein (GFP) reporter gene driven by nuclear factor of activated T cells (NFAT) activation via TCR signaling.

Mar 20, 2023 · In this study, we generated a novel platform cell line, CD4-2D3, in which GFP reporter was expressed by NFAT activation via TCR signaling, for correctly evaluating functional avidity of HLA class II-restricted TCRs.

Recombinant Jurkat T cells expressing both firefly luciferase and enhanced GFP (eGFP) under the control of NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway can be monitored by examining either firefly luciferase or eGFP expression. Background