Our results inform ongoing controversy about where XPA is bound within the NER bubble, provide structural insights into the molecular basis for malfunction of disease-associated XPA missense mutations, and contribute to understanding of the structure …

Nucleotide excision repair (NER) is a particularly important excision mechanism that removes DNA damage induced by ultraviolet light (UV). UV DNA damage results in bulky DNA adducts — these adducts are mostly thymine dimers and 6,4-photoproducts.

Xeroderma pigmentosum complementation group A (XPA) is an essential protein in the nucleotide excision repair (NER) pathway, the main DNA repair pathway responsible for the excision of bulky DNA lesions in both eukaryotic and prokaryotic cells.

Sep 1, 2024 · This review is concerned with a central driving force of the NER pathway: protein-protein interactions among the six core NER factors - XPC-RAD23B-CETN2, TFIIH, XPA, RPA, XPG, and ERCC1-XPF.

Jan 1, 2008 · Repair of bulky DNA adducts by the nucleotide excision repair (NER) pathway is one of the more versatile DNA repair pathways for the removal of DNA lesions.

Nucleotide excision repair (NER) clears genomes of DNA adducts formed by UV light, environmental agents, and antitumor drugs. Defects in NER cause the skin cancer-prone disease xeroderma pigmentosum, yet NER also counteracts the efficacy of antitumor agents.

Feb 2, 2011 · Although in vitro experiments show persistent binding of RPA, the current study adds mechanistic insights into how the participation of RPA in pre- and post-incision stages of NER regulates incision, and how impaired gap filling/sealing leads to an overall defect in photolesion repair.

Nov 18, 2014 · RPA avidly binds to single-stranded DNA (ssDNA) through multiple oligonucleotide/oligosaccharide-binding folds and coordinates the recruitment and exchange of genome maintenance factors to...

Dec 1, 2014 · RPA has differential dynamics during replication and the different steps of NER. UV-induced RPA accumulation in G0 cells represents post-incision NER events. RPA binding to the pre-incision complex is only visible in the absence of incision.

Feb 28, 2020 · The XPA protein functions together with the single-stranded DNA (ssDNA) binding protein RPA as the central scaffold to ensure proper positioning of repair factors in multi-protein nucleotide excision repair (NER) machinery.