T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.

For blunt ends or single base overhangs, incubate at 16°C overnight or room temperature for 2 hours (alternatively, high concentration T4 DNA Ligase can be used in a 10 minute ligation).

A thermotolerant variant of T4 DNA Ligase, Hi-T4™ DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini in duplex DNA or RNA and is designed to function at higher temperatures than wild type T4 DNA Ligase.

Use NEBioCalculator to calculate molar ratios. The T4 DNA Ligase Buffer should be thawed and resuspended at room temperature. Gently mix the reaction by pipetting up and down and microfuge briefly. For cohesive (sticky) ends, incubate …

On ice. T4 DNA Ligase should be added last. Note that the table shows a ligation using a molar ratio of 13 vector to insert for the indicated DNA sizes.) Use NEBioCalculator to calculate molar ratios.

10X reaction buffer supplied with T4 DNA Ligase. A 1X concentration assures optimal enzyme activity.

Jan 25, 2015 · Set up the reaction in a microcentrifuge tube on ice. Vector DNA (4 kb) 50 ng (0.020 pmol) Insert DNA (1 kb) 37.5 ng (0.060 pmol) Nuclease-free water to 20 μl. T4 DNA …

Immobilized T4 DNA Ligase (IM T4 DNA Ligase) is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg/ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry.

NEB’s T4 DNA Ligase has been referenced in peer-reviewed publications for decades, and is renowned for its consistent quality. For details on quality controls performed, see www.neb.com/ligasequality. End architecture (blunt-, T/A or sticky …

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.