NcoI - NEB
NcoI has a High Fidelity version NcoI-HF ® (NEB #R3193). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
NcoI-HF - NEB
NcoI-HF® has the same specificity as NcoI (NEB #R0193), but it has been engineered for reduced star activity. Not sensitive to CpG, dcm, or dam methylation. Protocols, Manuals & Usage
Enzyme Finder - NEB
Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang.
RJR REBASE Enz 1308 - NcoI
Prototype: NcoI Org #: 1910 Organism: Nocardia corallina Organism type: bacteria Organism source: ATCC 19070 Growth Temperature: 26 ° Experimental Evidence: biochemistry Exhibits star activity Enzyme gene cloned Enzyme gene sequenced Molecular Weight: 31346
NcoI - NEB
本酶提供高保真版本酶 NcoI-HF ® （NEB #R3193）。 高保真（HF）限制性内切酶在 rCutSmart 缓冲液中具有 100% 活性；统一缓冲液意味着更加直接、简化的样品处理过程。 HF 内切酶还会显著降低星号活性。 所有 HF 内切酶均符合省时酶（Time-Saver）标准，可在 5-15 分钟内酶切底物 DNA，也可实现过夜酶切，而且不会造成 DNA 降解。 HF 限制性内切酶在改造时将性能作为重要指标，可在更宽的条件下具有完全活性，最大限度地减少非特异性酶切产物，并且为实验设计 …
NcoI-HF - NEB
从 2021 年 4 月开始，NEB 将含有 BSA 的反应缓冲液转换为含有 重组白蛋白（rAlbumin） 的缓冲液，用于限制性内切酶和某些 DNA 修饰酶。 如需更多信息，请访问 www.neb.cn/BSA-free。 高保真（HF ®）限制性内切酶 具有与天然酶相同的特异性，但经过改造后显著降低星号活性，并使用统一的缓冲液（rCutSmart ™ 缓冲液）。 所有 HF 限制性内切酶均随附 6X 紫色凝胶上样染料。 经过改造的内切酶与天然内切酶相比较，虽然价格相同，但性能和性价比更高： 高保真（HF） …
What is the difference between NcoI-HF and NcoI? | NEB
Aug 28, 2011 · NcoI-HF is produced in E. coli from a strain that carries the cloned and modified NcoI gene from Nocardia carolina (ATCC 19070).
NcoI-HF® - NEB
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl. NcoI-HF® has the same specificity as NcoI (NEB #R0193), but it has been engineered for reduced star activity. Not sensitive to CpG, dcm, or dam methylation.
New England Biolabs, Inc. NcoI-HF – 5000 units | Fisher Scientific
Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and …
VWR
: New England Biolabs (NEB) An E.coli strain that carries the cloned and modified from Nocardia carolina (ATCC 19070). High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart® Buffer; single-buffer simplicity means more …
- Some results have been removed