Dec 22, 2022 · In this study, we aimed to gain further understanding of how ligand binding induces signalling of IL-23 receptor complexes using the proximity-based techniques of …

May 15, 2024 · Here we report cryo-electron microscopy structures of noradrenaline transporter in apo and substrate-bound forms, and as complexes with six antidepressants. The structures reveal a noradrenaline...

Jan 10, 2023 · In this study, NanoLuc Binary Technology (NanoBiT) was utilised to measure the formation of IL‐23 receptor complexes in the presence and absence of the cytokine, to assess if heteromers form on the cell surface prior to ligand engagement.

Nov 16, 2015 · NanoBiT is a complementation reporter designed for quantitative investigation of protein interaction dynamics under relevant physiological conditions. Benefiting from the small size and bright luminescence of NanoLuc, it provides detection at low intracellular concentrations with minimal steric interference on appended target proteins.

We investigated the possible involvement of the N-terminal extracellular domain (NTD) in dimerization/oligomerization and dimer-associated ligand binding by NanoLuc Binary Technology (NanoBiT).

The NanoBRET® TE Intracellular RAF Dimer Assays use NanoLuc® Binary Technology (NanoBiT), a structural complementation system comprised of a large luciferase subunit (LgBiT) and a small complementary peptide (SmBiT (q)), to evaluate the target engagement of RAF kinases in dimeric complexes.

Key results: NanoBiT measurements demonstrated that IL-23 receptor complexes formed to an equal degree in the presence and absence of ligand. NanoBRET measurements confirmed that these complexes bound IL-23 with a picomolar binding affinity.

Utilising a NanoBiT BRET binding assay, in which light generated from complemented receptors was used to excite fluorophore conju-gated IL-23 (IL23-TMR), it was possible to monitor binding interac-tions of IL-23 solely to pre-formed heteromers.

Dec 22, 2022 · In this study, we aimed to gain further understanding of how ligand binding induces signalling of IL‐23 receptor complexes using the proximity‐based techniques of NanoLuc Binary Technology (NanoBiT) and Bioluminescence Resonance Energy Transfer (BRET).

Dec 22, 2022 · In this study, we aimed to gain further understanding of how ligand binding induces signalling of IL-23 receptor complexes using the proximity-based techniques of …