Dec 6, 2018 · Our results demonstrate that 1H NMR spectroscopy provides a powerful method for the assessment of the cellular NAD metabolome. Nicotinamide adenine dinucleotide (NAD) and its phosphorylated form, NADP, are the major coenzymes of redox reactions in …

We have developed an NMR-based experimental approach suitable for the quantitative analysis of the NAD metabolome in human cells. The basic NMR characteristics (relaxation time, chemical shifts) were determined for all intermediates of NAD biosynthesis.

In the blood NMR spectra, the identities of the redox coenzymes NAD +, NADH, NADP +, and NADPH, the energy coenzymes, ATP along with ADP and AMP, and the antioxidants, GSH and GSSG, were established by combining 1D/2D NMR techniques, chemical shift databases, pH measurements, and spiking with authentic compounds. The measurement of important ...

Dec 6, 2018 · Here, we have developed an NMR-based experimental approach to detect and quantify NAD (P) and its biosynthetic intermediates in human cell extracts. Using this method, we have determined NAD, NADP, NMN and Nam pools in HEK293 cells cultivated in standard culture medium containing Nam as the only NAD precursor.

Nicotinamide adenine dinucleotide (NAD +) and its reduced form (NADH) are coenzymes employed in hundreds of metabolic reactions. NAD + also serves as a substrate for enzymes such as sirtuins, poly (ADP-ribose) polymerases (PARPs) and ADP-ribosyl cyclases.

Dec 6, 2018 · Here, we have developed an NMR-based experimental approach to detect and quantify NAD (P) and its biosynthetic intermediates in human cell extracts. Using this method, we have determined NAD,...

Feb 5, 2019 · The ability to simultaneously visualize absolute concentrations of CoA, acetyl-CoA, and endogenous CoA-S-S-G along with redox coenzymes (NAD +, NADH, NADP +, NADPH), energy coenzymes (ATP, ADP, AMP), antioxidants (GSH, GSSG), and a vast pool of other metabolites using a single 1D NMR spectrum offers a new avenue in the metabolomics field for ...

Aug 5, 2008 · The f erredoxin nicotinamide adenine dinucleotide p hosphate r eductase from Pseudomonas aeruginosa (pa -FPR) in complex with NADP + has been characterized by X-ray crystallography and in solution by NMR spectroscopy.

To confirm that purified recombinant wild-type IDH1 and IDH1 R132H enzymes contained copurifying NADP(H), we performed NMR experiments (Fig. 1 A and B). In the case of IDH1 R132H (Fig. 1B), NADPH, but not NADP +, copurified with the enzyme (see later).

Jan 1, 2001 · All H,H, H,P and several C,P coupling constants, including those between C-4' and the vicinal phosphorus atom, have been determined for NADP+, NADPH coenzymes and for a 4,4-dimer obtained from one-electron electrochemical reduction of NADP+.