Identification of NAD-RNA species and ADPR-RNA decapping in …
Nov 21, 2023 · We propose that the incorporation of NAD into RNA acts as a degradation marker for Saci-aCPSF2. In contrast, ADPR-RNA is processed by Saci_NudT5 into 5′-p-RNAs, providing another layer of...
NAD tagSeq for transcriptome-wide identification and ... - Nature
Aug 3, 2020 · Identification of the NAD + -capped RNAs (NAD-RNAs) could help to unveil the cap-mediated regulation mechanisms. We previously reported a method termed NAD tagSeq for genome-wide analysis of...
The uncharted territory of NAD + -capped RNA - Cell Press
Jan 18, 2024 · To expand the uncharted territory of RNA 5′-end NAD + capping, the sequencing of NAD-RNA at a single-base resolution is first required to demonstrate whether an adenosine, as a TSS, is necessary for NAD + capping.
NADcapPro and circNC: methods for accurate profiling of NAD
Apr 13, 2023 · In this study, we introduce two orthogonal methods to identify NAD-capped RNAs more precisely. The first, NADcapPro, uses copper-free click chemistry and the second is an intramolecular...
A Novel NAD-RNA Decapping Pathway Discovered by Synthetic Light-Up NAD ...
Mar 28, 2020 · In addition to the canonical 5'-N7-methyl guanosine cap in eukaryotes, the ubiquitous redox cofactor nicotinamide adenine dinucleotide (NAD) was identified as a new 5'-RNA cap structure in prokaryotic and eukaryotic organisms.
ONE-seq: epitranscriptome and gene-specific profiling of NAD-capped RNA ...
The hub metabolite, nicotinamide adenine dinucleotide (NAD), can be used as an initiating nucleotide in RNA synthesis to result in NAD-capped RNAs (NAD-RNA). Since NAD has been heightened as one of the most essential modulators in aging and various ...
The uncharted territory of NAD + -capped RNA - PubMed
The critical redox cofactor NAD + was recently reported to serve as an RNA cap in both eukaryotes and prokaryotes. However, its reversible regulation and biological functions remain unclear. Here, we provide insights into its discovery, capping and decapping mechanisms, for further discove …
“NAD-capQ” detection and quantitation of NAD caps - PMC
RNA 5′ cap structures comprising the metabolic effector nicotinamide adenine dinucleotide (NAD) have been identified in diverse organisms. Here we report a simple, two-step procedure to detect and quantitate NAD-capped RNA, termed “NAD-capQ.”
NAD-capped RNAs - a redox cofactor meets RNA - PubMed
In the past few years novel technologies and methods have characterized NAD as a cap-like RNA structure and enabled the investigation of NAD-capped RNAs (NAD-RNAs) in a physiological context. We highlight the identification of NAD-RNAs as well as the regulation and functions of this epitranscriptomic mark in all domains of life.
NAD-capped RNAs – a redox cofactor meets RNA - ScienceDirect
Feb 1, 2023 · In the past few years novel technologies and methods have characterized NAD as a cap-like RNA structure and enabled the investigation of NAD-capped RNAs (NAD-RNAs) in a physiological context. We highlight the identification of NAD-RNAs as well as the regulation and functions of this epitranscriptomic mark in all domains of life.