Using NSG mice as human xenograft recipients, it is now possible to grow almost all types of primary human tumors in vivo, including most solid tumors and hematological malignancies that maintain characteristics of the primary tumor in the patient.

Sep 15, 2021 · Because mouse prolactin fails to activate hPRL receptors, we developed a prolactin-humanized Nod-SCID-IL2Rγ (NSG) mouse (NSG-Pro) with physiological hPRL levels. Here, we show that NSG-Pro mice facilitate establishment of therapy-naïve, estrogen-dependent PDX tumors that progress to lethal metastatic disease.

Aug 1, 2020 · Technical advances in genome sequencing and the implementation of next-generation sequencing (NGS) in clinical oncology have paved the way for individualizing cancer patient therapy based on molecular profiles.

To demonstrate the differences, we implanted oral and pancreatic tumors in NSG and hu-BLT mice and then compared the dynamics of tumor growth, rate of expansion, and differentiation among these two mouse models. Tumors grew much faster, and larger tumors formed in NSG mice when compared to hu-BLT mice.

NSG™ is one of the most immunodeficient mouse strains described to date. Here's why: The NOD genetic background contains alleles that reduce the function of the innate branch of the immune system. Consequently, macrophages and dendritic cells are defective.

The unparalleled immune deficiency of NSG mice has made them a preferred host for establishing and passaging the human primary tumors in our Patient-Derived Xenograft (PDX) Resource, which now contains more than 200 established, low-passage models.

NSG mice engrafted with human PBMC typically develop overt signs of acute GvHD beginning at 3–4 weeks post injection . A number of variants of the parental NSG, NRG, and NOG lines have been developed that eliminate murine MHC and delay onset of GvHD.

Aug 13, 2019 · The severe immunodeficiency of NOD.Cg-PrkdcscidIl2rgtm1Wjl (NSG) mice, a widely used strain for generating patient-derived xenografts from primary tumors, impairs drug metabolism and reduces ...

Previously developed and widely used NOD-scid IL2rγ null (NSG) mice support enhanced engraftment of many primary human tumors. However, NSG mice have a 2-bp deletion in the coding region of the hemolytic complement (Hc) gene, and it is not possible to evaluate CDC activity in NSG mice.

To identify improved mouse models for breast cancer growth and spontaneous metastasis, we examined growth and metastasis of both estrogen receptor positive (T47D) and negative (MDA-MB-231, SUM1315, and CN34BrM) human breast cancer cells in nude and NSG mice.