Nucleolus assembly impairment leads to two-cell transcriptional ...
May 18, 2023 · LoNA tethers nucleophosmin (NPM1), a GC-enriched protein, to fibrillin (FBL), a DFC-enriched protein, and drives the formation of the compartmentalized nucleolus via …
Phase separation-competent FBL promotes early pre-rRNA
May 14, 2024 · EGFP-tagged proteins (FBL, MD, GAR, NPM1 and PLD-MD) were cloned into pET-28a for expression in Escherichia coli BL21 (DE3) (AlpalifeBio KTSM104L), induced with …
Germline NPM1 mutations lead to altered rRNA 2’-O-methylation …
We identify NPM1 germline mutations in DC patients presenting with BMF, and demonstrate that they are deficient in snoRNA binding. Mice harboring a DC germline NPM1 mutation …
Controlling the material properties and rRNA processing function …
Aug 9, 2019 · Two key nucleolar proteins that have received the most attention are nucleophosmin (B23/NPM1) and fibrillarin (FBL). NPM1 is a highly expressed nucleolar protein …
Advances in the structure and function of the nucleolar protein ...
Fibrillarin (FBL) is a highly conserved and well-researched nucleolar protein found in eukaryotes. Its presence was first identified in 1985 through protein immunoblotting analyses using …
专家点评Mol Cell | 陈玲玲组首次提出相分离促进新生成rRNA前体 …
FBL是rRNA前体加工过程中的一种重要的加工因子，包含了一个富含甘氨酸和精氨酸的内在无序区域（intrinsically disordered regions，IDRs）结构域（GAR）以及一个甲基转移酶结构 …
ZNF692 regulates nucleolar morphology by interacting with NPM1 …
Jun 21, 2023 · In this study, we demonstrated that the nucleolar protein ZNF692 directly interacts with nucleophosmin 1 (NPM1). Knocking down ZNF692 resulted in the nucleolar redistribution …
The interaction between nucleophosmin/NPM1 and the large …
Jan 1, 2021 · We demonstrated that NPM1 directly interacts with the large ribosomal subunits and maintains them in the nucleolus. Ectopically localized NPM1 efficiently recruits only the large …
Nucleolus: A Central Hub for Nuclear Functions - ScienceDirect
Aug 1, 2019 · The key nucleolar proteins FBL, nucleophosmin 1 (NPM1), and NOPP140 retain their mobility and evade immobilization, highlighting the specificity of this nucleolar sequestration.
The nucleolus functions as a phase-separated protein quality …
Jul 11, 2019 · Transient associations with nucleolar proteins such as NPM1 conferred low mobility to misfolded proteins within the liquid-like GC phase, avoiding irreversible aggregation. …