May 20, 2010 · We developed a new approach using the amino-acid-based nanoparticle HB-OLD7 for local delivery of siRNA targeting NOX2 to the arterial wall. siRNA–nanoparticle complexes were...

Oct 1, 2013 · In this report, we successfully encapsulated Nox2-specific siRNA into the acid-sensitive polyketal PK3 particle for silencing of the Nox2 gene in cardiac macrophages and inactivation of NADPH oxidase.

Here, we demonstrate acid-degradable polyketal particles as delivery vehicles for Nox2-siRNA to the post-MI heart. In vitro, Nox2-siRNA particles are effectively taken up by macrophages and significantly knockdown Nox2 expression and activity.

In one study, siRNA against NOX2 inhibited both ROS production and p38-MAP kinase-dependent proliferation in an endothelial cell line and in primary endothelial cells, while overexpression of NOX2 increased both responses (221).

Dec 9, 2011 · PK3-siNox2 treated cells exhibited a 41% reduction in activity, with no significant changes seen with scrambled siRNA or empty particle treatment. Currently, the therapeutic potential of the Nox2-siRNA particles is being evaluated in a …

To dissect out the relative contributions of these Nox homologues, we utilized adenoviral-mediated delivery of siRNA to the SFO of mouse brain to induce stable and localized knockdown of Nox2 or Nox4.

Nov 19, 2010 · Based on our studies, Nox2-based NADPH oxidase (gp91 phox)-induced ROS may have important roles on proliferation in HCB induced EPCs by VEGF stimulation. Furthermore, Nox2 siRNA transfection into HCB derived EPC down-regulated intracellular ROS production and pERK expression.

We have developed a new approach using the amino-acid-based nanoparticle HB-OLD7 for local delivery of siRNA targeting NOX2 to the arterial wall. siRNA-nanoparticle complexes were transferred into regional carotid artery walls after angioplasty in an atherosclerotic rat model.

Aug 1, 2011 · Furthermore, the critical roles of Nox2-derived ROS in mediating PPARγ signaling were confirmed by (1) knocking down Nox2 using siRNA, (2) using cells isolated from Nox2 knockout mice, and (3) treating Nox2 knockout mice with GW1929.

To dissect out the functional importance and unique roles of these Nox enzymes in the pressor and dipsogenic effects of central Ang-II, we developed adenoviral vectors expressing small interfering RNA to selectively silence Nox2 or Nox4 expression in the subfornical organ.