NOX2 knockout mice displayed significantly less endothelial ROS production and significantly higher endothelium-dependent relaxation than wild-type mice . Human CGD patients manifest increased vascular NO and increased vasodilation, providing a direct link between NOX2 and endothelial function .

Jun 8, 2020 · NOXO1 is consistently upregulated in two models of lung emphysema, Cybb (also known as NADPH oxidase 2, Nox2)-knockout mice and wild-type mice with tobacco-smoke-induced emphysema, and in human...

Of the catalytic NADPH oxidase subunits (NOX), NOX4 is the most widely distributed isoform. We provide here a critical review of the currently available experimental tools to assess the role of NOX and especially NOX4, i.e. knock-out mice, siRNAs, …

Apr 1, 2016 · Knockout mice models are now available for all mammalian Noxs and their regulators, except for Nox5, which is not expressed in mice. Experiments on gene-deficient animals have helped to understand the physiological function of Nox3 and confirmed the essential role of Duox2 in thyroid hormone synthesis.

With use of Nox isoform–specific knockout mice, functional analysis revealed Nox2 as the predominant isoform. In human islets, NOX2 colocalized with both insulin granules and endosome/lysosome membranes.

Jul 5, 2020 · Nox2 and Nox4 knockout mice had increased basal DNA damage, concealing possible angiotensin II-induced increases. In conclusion, in the kidney, Nox1 seemed to play a role in angiotensin II-induced DNA damage.

Oct 26, 2020 · Here, we investigate why NOX2 knockout mice show no NOX2 activity but express NOX2 mRNA and protein. Methods and results: Oxidative burst (NOX2-dependent formation of ROS) was measured by L-012-based chemiluminescence and was largely absent in whole blood of NOX2 knockout mice.

Jul 22, 2019 · Oxidative stress plays an important role in aging-related neurodegeneration. This study used littermates of WT and Nox2-knockout (Nox2KO) mice plus endothelial cell-specific human Nox2 overexpression-transgenic (HuNox2Tg) mice …

One source is NADPH oxidase, which is especially important for superoxide radical production. Nox2 is a primary regulatory subunit of NADPH oxidase. In the present study, we examined the role of ROS and NADPH oxidase in ischemic preconditioning (IP)-mediated cardioprotection by using Nox2(-/-) mice.

Oct 13, 2024 · This study investigated the suitability of p22phox protein as a marker of Nox activity. To avoid contamination by phagocytic p22phox, we developed global Cybb (encoding Nox2)-knockout mice and analyzed p22phox stability and the expression profiles of Nox proteins in lysates of various tissues.