Therefore, we propose NLS-tagging as an effective and fast alternative strategy to the common N- or C-terminal tag integration methods for the characterization of protein complexes formed by TFs and the identification of their DNA binding regions.

May 22, 2021 · Nuclear localization signals (NLS) are generally short peptides that act as a signal fragment that mediates the transport of proteins from the cytoplasm into the nucleus.

A nuclear localization signal or sequence (NLS) is an amino acid sequence that 'tags' a protein for import into the cell nucleus by nuclear transport. [1] Typically, this signal consists of one or more short sequences of positively charged lysines or arginines exposed on the protein surface. [ 1 ]

Specifically, we will highlight the most significant advancements in NLS-tagging for various therapeutic platforms, with a particular focus on structural elements such as NLS tag densities and orientations, conjugation mode, and diseased cellular environments.

Dec 1, 2014 · We demonstrate the effectiveness of this approach with the transcription factors Fli-1 and Irf2bp2, which cannot be tagged at their extremities without loss of function. This resulted in the identification of novel proteins partners and a new hypothesis on the contribution of Fli-1 to hematopoiesis. © The Author (s) 2014.

We report here six different NLS classes that specifically bind to distinct binding pockets of importin α. By screening of random peptide libraries using an mRNA display, we selected peptides bound by importin α and identified six classes of NLSs, including three novel classes.

Jul 14, 2014 · Here we report the development of LINuS, a light-inducible, fully reversible and genetically encoded nuclear localization signal (NLS) based on a single, small tag, which can be fused to proteins...

Jan 1, 2000 · Proteins enter the nucleus from the cytoplasm through a 25-nm active channel by a facilitated process that often requires the presence of a nuclear localization signal (NLS) on the transported protein as well as soluble transport factors.

A signal sequence encoded in a protein that causes nuclear import is recognized as the nuclear localization signal (NLS), whereas the sequence that causes nuclear export is called the nuclear export signal (NES).

Here, we summarized the types of NLS, focused on the recently reported related proteins containing nuclear localization signals, and briefly summarized some mechanisms that do not depend on nuclear localization signals into the nucleus.