Apr 30, 2001 · Concomitant induction of NFAT and AP-1 requires concerted activation of two different signaling pathways: calcium/calcineurin, which promotes NFAT dephosphorylation, nuclear translocation and...

We show that Compound 10 disrupts the NFAT:AP-1 interaction at several but not all composite NFAT:AP-1 sites, without inhibiting the binding of NFAT or Fos-Jun dimers to DNA; and that it exerts these inhibitory effects by binding in a sequence-selective manner to DNA.

Apr 30, 2001 · The four calcium-regulated transcription factors of the NFAT family act synergistically with AP-1 (Fos/Jun) proteins on composite DNA elements which contain adjacent NFAT and AP-1 binding sites, where they form highly stable ternary complexes to regulate the expression of diverse inducible genes.

May 14, 2019 · Envisioning a strategy for selective modulation of the immune response, we designed a FRET-based high-throughput screen to identify compounds that disrupt the NFAT:AP-1:DNA complex. We screened ∼202,000 small organic compounds and …

The effects of the AP-1 transcription factors, associated with the immune system, are largely mediated through combinatorial regulation with the NFAT, a calcium/calcineurin pathway-dependent transcription factor. The transcription factors of the NFAT family are key regulators of T cell activation [43].

Jun 1, 2005 · In activated T cells, NFAT proteins synergize with activator protein 1 (AP1) transcription factors at composite sites that are located in the promoters and enhancers of many cytokine genes.

Nov 10, 2021 · ICOS signaling upregulates Bcl6 and induces an AP-1–independent NFAT program in primary T cells. Using lupus-prone mice, we demonstrate that genetic disruption or pharmacologic inhibition of NFAT signaling specifically impacts Tfh cell maintenance and leads to amelioration of autoantibody production and renal injury.

Here, we show that both AP-1 and NFAT transcriptional activities are not inducible in the majority of DP cells but that during the differentiation of DP cells to the mature single-positive stage, thymocytes regain this inducibility.

Feb 1, 2007 · Taken together, our data show that Ral is activated upon BCR stimulation and mediates BCR-controlled activation of AP-1 and NFAT transcription factors. These findings suggest that Ral plays an important role in B cell development and function.

A ®fth member of the NFAT family, NFAT5, controls the cellular response to osmotic stress, by a mechanism that requires dimer formation and is independent of calcineurin or of interaction with...