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NEBcutter incorporates everything that is known about the methylation sensitivity of any of the enzymes displayed when they overlap say a Dam site or a Dcm site as well as CpG, EcoK and …

Note that if a GenBank file is used that contains a circular sequence NEBcutter will pick that up automatically. "Enzymes to use" i) "NEB enzymes" is the default and encompasses all …

NEBcutter : What's new in Version 2.00: General: Up to 40 custom oligonucleotide sequences can be specified. Most display colors can be customized. Options to include Type I and III …

Pick your enzyme or enter a name in the box. Only the enzymes that cut the sequence and match your selection of availability (all) are shown. Non-NEB neoschizomers are not listed, but you …

Published restriction maps generated by NEBcutter should cite: Vincze, T., Posfai, J. and Roberts, R.J. NEBcutter: a program to cleave DNA with restriction enzymes

Pick your enzyme or enter a name in the box. Only the enzymes that cut the sequence and match your selection of availability (all commercial) are shown.

NEBcutter - Custom Digest