Here we describe the first synthesis of a site-specific NHMG-containing oligonucleotide via the 2′-fluorination strategy. In addition, we present kinetic and structural assessment of human DNA polymerase η (polη) bypassing NHMG generated by an N -phenyl nitrogen mustard.

Here, we present m 7 G Mutational Profiling sequencing (m 7 G-MaP-seq), which allows high throughput detection of m 7 G modifications at nucleotide resolution. In our method, m 7 G modified positions are converted to abasic sites by reduction with sodium borohydride, directly recorded as cDNA mutations through reverse transcription and sequenced.

Sep 13, 2019 · Here, we have established m 7 G individual-nucleotide-resolution cross-linking and immunoprecipitation with sequencing (m 7 G miCLIP-seq) to specifically detect internal mRNA m 7 G...

Nov 18, 2022 · Here, we introduce m 7 G-quant-seq, a quantitative method that accurately detects internal m 7 G sites in human cytoplasmic tRNAs at single-base resolution. The efficient chemical reduction and mild depurination can almost completely convert internal m 7 G sites into RNA abasic sites (AP sites).

N7-methylguanosine (m7G) is one of the most common post-transcriptional epigenetic modifications. Different m7G methyltransferases (writers) load the m7G-cap at the 5’-terminal or inside the RNAs.

Nov 18, 2019 · Methylation of guanosine on position N7 (m7G) on internal RNA positions has been found in all domains of life and have been implicated in human disease. Here, we present m7G Mutational Profiling sequencing (m7G-MaP-seq), which allows high throughput detection of m7G modifications at nucleotide resolution.

A simple and sensitive reversed-phase high-performance liquid chromatographic (HPLC) method for the determination of N7G from DNA using ultraviolet detection is described. DNA samples having been exposed to HN2 treatment were hydrolyzed and preseparated from high-molecular-mass material by filtration using a molecular mass cut-off of 3000.

Jul 27, 2022 · tRNA modification is closely associated with tumorigenesis and its role is comparable with that of other RNA modifications. m 7 G is a universal tRNA modification in prokaryotes and eukaryotes that is catalyzed by the compounds methyltransferase-like protein-1 (METTL1) and WD repeat domain 4 (WDR4).

We have developed a quantitative HPLC-ESI+-MS/MS method for measuring racemic and meso forms of bis-N7G-BD in DNA extracted from tissues of BD-exposed laboratory animals. In our approach, bis-N7G-BD adducts are released from DNA as free bases by neutral thermal hydrolysis, purified by solid-phase extraction, and subjected to HPLC-ESI+-MS/MS ...

Jul 16, 2020 · In his Letter in this issue of Molecular Cell, Vinther (2020) asserts that in Pandolfini et al. (2019), we misinterpreted some of our data mapping m 7 G to let-7, and he claims that our sequencing-based methods used to detect m7G within miRNAs are flawed.