5,10-Methylenetetrahydrofolate (N5,N10-Methylenetetrahydrofolate; 5,10-CH2-THF) is cofactor in several biochemical reactions. It exists in nature as the diastereoisomer [6R]-5,10-methylene-THF. As an intermediate in one-carbon metabolism, 5,10-CH 2 -THF converts to 5-methyltetrahydrofolate, 5-formyltetrahydrofolate, and methenyltetrahydrofolate.

5,10-Methenyltetrahydrofolate (5,10-CH=THF) is a form of tetrahydrofolate that is an intermediate in metabolism. 5,10-CH=THF is a coenzyme that accepts and donates methenyl (CH=) groups. It is produced from 5,10-methylenetetrahydrofolate by either a NAD+ dependent methylenetetrahydrofolate dehydrogenase , or a NADP+ dependent dehydrogenase . [ 1 ]

Jun 11, 2018 · MTHFR catalyzes the physiologically irreversible reduction of 5,10-methylene-tetrahydrofolate (CH 2 -THF) to 5-methyl-tetrahydrofolate (CH 3 -THF), a reaction requiring FAD as a cofactor and...

5,10-Methylene-THF is a metabolite found in or produced by Saccharomyces cerevisiae. 2- [ [4- [ (6 aR)-3-amino-1-oxo-2,5,6,6 a,7,9-hexahydroimidazo [1,5-f]pteridin-8-yl]benzoyl]amino]pentanedioic acid.

Jun 11, 2018 · Our 2.5 Å resolution crystal structure of human MTHFR reveals a unique architecture, appending the well-conserved catalytic TIM-barrel to a eukaryote-only SAM-binding domain.

10-formyl tetrahydrofolate is a key metabolite in C1 carbon metabolism, arising through the action of formate-tetrahydrofolate ligase (FTL) and/or 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase (DHCH).

Mar 12, 2019 · Methylenetetrahydrofolate reductase (MTHFR) catalyzes the irreversible conversion of 5,10-methylene-tetrahydrofolate (THF) to 5-methyl-THF, thereby committing one-carbon units to the methionine...

Methylenetetrahydrofolate reductase (MTHFR) deficiency is a rare autosomal recessive metabolic disorder with an unknown prevalence and is the most common genetic cause of elevated levels of homocysteine in the plasma (hyperhomocysteinemia). It is …

Oct 13, 2023 · Methylenetetrahydrofolate dehydrogenase–cyclohydrolase (FolD) is a dual-function enzyme that converts N 5, N 10 -methylene-THF into N 10 -formyl-THF in a two-step reaction.

(E) Schematic representation of the N5,N10-methylene-THF synthase reaction. The bound THF attacks the iminium ion via the nucleophilic N10 group with concomitant proton abstraction by...