Apr 1, 2007 · To examine the effect of knocking out the myostatin gene after muscle has matured, we generated mice in which myostatin exon 3 was flanked by loxP sequences (Mstn [f/f]) and …

Jan 11, 2010 · Here, we used Mstn gene-targeted mice in which the third exon is flanked by loxP sites (Mstn fl/fl) to permit tissue-specific deletion with an appropriate cre-expressing line.

Nov 20, 2020 · Myostatin (MSTN) is a secreted signaling molecule that normally acts to limit skeletal muscle growth (for review, see ref. 1). Mice lacking MSTN exhibit dramatic increases …

Aug 8, 2021 · Myostatin (MSTN) is a transforming growth factor-β (TGF-β) superfamily member that normally acts to limit skeletal muscle mass (1). Mice lacking MSTN exhibit dramatic …

Myostatin was undetectable in muscle within 2 wk after Cre recombinase activation in 4-month-old male mice with floxed myostatin genes.

Myofibers are clearly the predominant source of circulating MSTN protein, as plasma MSTN levels have been shown to be severely reduced in Mstnflox/flox, Myl1-cre mice, in which Mstn has …

Jul 7, 2019 · Mstn−/− mice are viable and have increased skeletal muscle weight as well as increased heart weight and heart weight to tail length, but they show no increase in heart …

Jun 18, 2024 · In our study, we specifically knocked out MSTN in brown adipose tissue (BAT) from mice (MSTN ΔUCP1) and found that the mice gained more weight than did controls when …

May 7, 2014 · To overexpress myostatin conditionally, we created CAGG-Mstn mice by random integration of a flox-βGeo-flox-myostatin-IRES-EGFP cassette into the genome of embryonic …

Mar 15, 2016 · Analysis of posteriorly located vs. anteriorly located muscles of these mice revealed clear differential effects indicative of an important paracrine role for MSTN in …