CellLight Mitochondria-GFP, BacMam 2.0, is a fusion construct of the Leader sequence of E1 alpha pyruvate dehydrogenase and emGFP, providing accurate and specific targeting to cellular mitochondria-GFP.

Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells. Karbowski et al J Cell Biol. 2004 Feb 16. 164 (4):493-9. ( How to cite ) This material is available to academics and nonprofits only.

Jul 14, 2017 · Mito-GFP contains the mitochondrial localization sequence from cytochrome oxidase VIII which targets GFP to the mitochondria and has been used to study mitochondrial distribution in the...

Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Mitochondria Cyto-Tracer, pCT-Mito-GFP (CMV) fuses a Cox8 tag to copGFP, resulting in copGFP-labeling of mitochondria.

Plasmid pLV-mitoGFP from Dr. Pantelis Tsoulfas's lab contains the insert mitoGFP and is published in Hum Mol Genet. 2013 Jan 24. This plasmid is available through Addgene.

Jun 19, 2019 · We silenced DRP1 in wild-type and dynamin triple-knockout cells, and labelled mitochondria with mito-GFP.

In this chapter, we detail the use of advanced confocal microscopy and mitochondrial matrix-targeted photoactivatable green fluorescent protein (mito-PAGFP) for the investigation of mitochondrial dynamics. We focus on direct visualization and quantification of mitochondrial fusion and mitochondrial network complexity in living mammalian cells.

Jul 20, 2012 · Mitochondrial fusion plays an essential role in mitochondrial calcium homeostasis, bioenergetics, autophagy and quality control. Fusion is quantified in living cells by photo-conversion of matrix targeted photoactivatable GFP (mtPAGFP) in a subset of mitochondria.

Plasmid pAcGFP1-mito from Dr. Yusuke Ohba's lab contains the insert AcGFP with mitochondria-targeting sequence and is published in Cell Struct Funct. 2019 Dec 26;44 (2):183-194. doi: 10.1247/csf.19028. Epub 2019 Nov 15. This plasmid is available through Addgene.

May 10, 2021 · Inactivation of an essential macroautophagy gene, ATG5, in the mito-GFP line (atg5-1::mito-GFP) led to the accumulation of depolarized mitochondria in DNP-treated as well as untreated roots, indicating that removal of depolarized …