MicroRNA sequencing (miRNA-seq), a type of RNA-Seq, is the use of next-generation sequencing or massively parallel high-throughput DNA sequencing to sequence microRNAs, also called miRNAs. miRNA-seq differs from other forms of RNA-seq in that input material is often enriched for small RNAs. miRNA-seq allows researchers to examine tissue ...

Small RNA sequencing (RNA-Seq) is a technique to isolate and sequence small RNA species, such as microRNAs (miRNAs). Small RNA-Seq can query thousands of small RNA and miRNA sequences with unprecedented sensitivity and dynamic range.

May 27, 2021 · Small RNA-seq enables genome-wide profiling and analysis of known, as well as novel, miRNA variants. Moreover, its high sensitivity allows for profiling of low input samples such as liquid biopsies, which have now found applications in diagnostics and prognostics.

Mar 1, 2021 · We describe quality assessment of raw sequencing data files, reference-based alignment, counts generation for miRNA expression levels, and novel miRNA discovery.

Select organism, chromosome and start and end coordinates. Leave the start/end boxes blank to retrieve all miRNAs on the selected chromosome. Comments or questions? Email [email protected].

miRNA sequencing is a powerful technique for comprehensive analysis of miRNA expression and the discovery of novel miRNAs. Here are some commonly used miRNA sequencing methods: Small RNA sequencing (sRNA-seq) constitutes a prevalent methodology in the sequencing of …

Discover how researchers at THI are using QIAGEN’s miRNA-seq solution for analyzing miRNA expression in plasma-derived exosomes from lung transplant patients. Unique molecular indices (UMIs) increase accurate quantification of individual miRNA molecules.

Apr 18, 2012 · Three main approaches are currently well established for miRNA profiling: quantitative reverse transcription PCR (qRT-PCR), hybridization-based methods (for example, DNA microarrays) and...

MicroRNA-seq allows researchers to characterize and quantify the expression and prevalence of the small non-coding RNA moleccules known as microRNA. These molecules may play an important role in diseases, and significant effort is underway to understand their effects across a variety of tissue types and cells.

microRNAs (miRNAs) are small RNA molecules of 17 to 24 bp that play an important role in the regulation of gene expression by modulating translation and stability of mRNA. Next generation sequencing technologies have enabled sequencing of …