Jun 2, 2020 · MicroRNAs (miRNAs) associated with Argonaute proteins (AGOs) regulate gene expression in mammals. miRNA 3’ ends are subject to frequent sequence modifications, which have been proposed to affect...

In the genome of Arabidopsis thaliana (Ath), 10 different AGO proteins are encoded and the sorting decision, which miRNA associates with which AGO protein, was reported to depend exclusively on the identity of the 5′-sequence position of mature miRNAs.

MicroRNAs (miRNAs) are small noncoding RNAs that function in literally all cellular processes. miRNAs interact with Argonaute (Ago) proteins and guide them to specific target sites located in the 3′-untranslated region (3′-UTR) of target mRNAs leading to translational repression and deadenylation-induced mRNA degradation.

In this work we compared the total miRNA content in AGO1 and AGO2 immunoprecipitates obtained from MCF7 adenocarcinoma cells using TaqMan Low Density miRNA Arrays and successfully verified selected miRNAs with qPCR.

Nov 25, 2015 · microRNAs (miRNAs) act as sequence-specific guides for Argonaute (AGO) proteins, which mediate posttranscriptional silencing of target messenger RNAs. Despite their importance in many biological...

Here, we present a technique called Argonaute-RNA Immunoprecipitation (AGO-RIP) which takes advantages of the fact that miRNAs and their targets are directly bound by the Argonaute protein family. With this approach quantitative, genome-wide analysis of miRNA targets is possible.

Jan 15, 2024 · Target-directed miRNA degradation (TDMD) occurs when trigger RNAs bind the miRNA–AGO complex and recruit the ZSWIM8 E3 ubiquitin ligase, leading to AGO ubiquitination and proteolysis and subsequent miRNA destruction.

miRNA结合靶点的效率对于理解miRNA的作用过程和作用机制大有裨益，但是对于miRNA-AGO复合物与靶标转录本之间结合亲和力的检测一直没有很好的方法。 近日，Whitehead研究所 David P. Bartel 研究组在 Science 发表文章 The biochemical basis of microRNA targeting efficacy，对RNA bind-n-seq（RBNS）测序进行优化，用于 测量miRNA-AGO复合体与所有小于12nt靶标结合的相对亲和力的大小，为定量测量miRNA对于基因调控网络的结合效率提供了重要的生物化学基础。

Feb 28, 2024 · MicroRNAs (miRNAs) guide Argonaute (AGO) proteins to bind mRNA targets. Although most targets are destabilized by miRNA-AGO binding, some targets induce degradation of the miRNA instead. These special targets are also referred to as trigger RNAs.

Sep 20, 2018 · Nuclear localization of AGO proteins allows miRNA-mediated gene silencing to bypass the usual targeting constraint and expand its effective target sequence space to include CDS and introns in addition to the 3′ UTR.