Here, based on current understanding of the miRNA maturation pathway, we discuss the principles of different shRNA design (pre-miRNA-like, pri-miRNA-like and Ago-shRNA) with an emphasis on the RNA structure.

Dec 26, 2013 · Short hairpin RNA (shRNA) technology enables stable and regulated gene repression. For establishing experimentally versatile RNAi tools and minimizing toxicities, synthetic shRNAs can be embedded into endogenous microRNA contexts.

Jul 1, 2013 · This protocol describes the creation of miR-30-based short hairpin RNA (shRNA) cassettes that are compatible with a number of standard vector systems. The principles outlined here can also be easily applied to other miRNA scaffolds or to simple snapback shRNAs.

6 days ago · Our Lentivirus miR30-Based shRNA Knockdown vectors incorporate an optimized micro-RNA system and are derived from the third-generation lentiviral vector system.

Aug 23, 2006 · We have constructed first-generation short hairpin RNA (shRNA) libraries modeled after precursor microRNAs (miRNAs) and second-generation libraries modeled after primary miRNA transcripts (the...

6 days ago · Our regular plasmid miR30-based shRNA knockdown vector incorporates an optimized micro-RNA system for transient knockdown of target gene (s) and is optimized for high copy number replication in E. coli and high-efficiency transfection.

Here, using the potential therapeutic target BCL11A, we demonstrate that pol III-driven shRNAs lead to significantly increased knockdown but also increased cytotoxcity in comparison to pol II-driven miRNA adapted shRNAs (shRNA (miR)) in multiple hematopoietic cell lines.

16 hours ago · The plasmids and reagents utilized included: Plasmids and Constructs: shRNA Plasmids: The pgLV3/H1/GFP + Puro vector (GenePharma, Shanghai, China) was used to deliver shRNA targeting miR-130a-5p or a non-targeting control. The same vector was also employed to express human TFPI2 cDNA (OE-TFPI2) or a scrambled control (OE-NC).

Feb 24, 2022 · In this study, we develop miR‐AB, a new microRNA‐based shRNA (shRNAmir) backbone for simplified, cost‐effective, and error‐proof production of shRNAmirs.

Dec 12, 2023 · Here, we describe a microRNA (miRNA)-based multiplex shRNA platform obtained by combining highly efficient miRNA scaffolds into a chimeric cluster, to deliver up to four shRNA-like sequences.