As of 2019, the live colony of Stock No. 007001 has been backcrossed a total of at least 11 generations (the last seven backcross generations were onto C57BL/6J).

In an effort to disambiguate previous data and to provide a resource to aid appropriate experimental design, here we summarise what is known about Cre recombinase activity in the most widely used endothelial-specific Cre and Cre/ERT2 mouse models.

024709 B6.Cg-Tg (Ins1-cre/ERT)1Lphi/J These MIP1-CreERT mice express a transgene with tamoxifen inducible Cre recombinase in pancreatic beta cells.

016261 C57BL/6-Tg (Nes-cre/ERT2)KEisc/J <i>Nestin-Cre/ERT2</i> transgenic mice express tamoxifen-inducible Cre recombinase in adult and developing mouse brain, including neuronal and glial cell precursors.

The double fluorescent marker system allows visualization of both recombined and non-recombined cells. We used the mT/mG mouse line to evaluate Cre-ERT2 activity in vivo. In addition, we also utilized Ece1flox/flox mice to confirm Cre activity in …

Successful gene targeting with Cre and Cre/ERT2-loxP is critically determined by the precise expression pattern of Cre recombinase. A single Cre-mediated excision event is passed on during cell division to all subsequent daughter cells. In this …

Transgenic mice were identified by using PCR and Southern blotting. Expression of Cre-ERt fusion gene was analyzed in the liver, kidney, brain and lung from F1 generation transgenic mice at 8 weeks of age by reverse transcription (RT)-PCR.

One approach to regulate Cre activity utilizes a mutant estrogen hormone-binding domain (ERT) to keep Cre inactive unless the non-steroidal estrogen analog 4-hydroxytamoxifen (OHT) is present. Here we describe a mouse strain expressing Cre-ERT from the ubiquitously expressed ROSA26 (R26) locus.

We examined the use of ERT2-iCre-ERT2 (Cre2ERT2), a tamoxifen-regulated form of Cre that has been described to have a background activity lower than that of other tamoxifen-regulated Cre constructs, for establishing performant conditional deleter mouse lines.

To introduce temporal control in genetic experiments targeting the endothelium, we established a mouse line expressing tamoxifen-inducible Cre-recombinase (Cre-ERT2) under the regulation of the vascular endothelial cadherin promoter (VECad).