meRIP-qPCR - 知乎 - 知乎专栏
RNA甲基化免疫共沉淀 （Methylated RNA Immunoprecipitation，MeRIP）基于m6A特异性抗体识别并结合mRNA上的m6A的原理，以 RNA免疫共沉淀 方法富集甲基化修饰片段为基础，并通 …
• Real-time PCR thermal cycler • NanoDrop™ or spectrophotometer, or Bioanalyzer
An experimental workflow for identifying RNA m6A alterations in ...
A schematic diagram showing the meRIP-seq procedures, including sample preparation, total RNA extraction and mRNA isolation, immunoprecipitation, sequencing and bioinformatic …
MeRIP-seq for Detecting RNA methylation: An Overview
MeRIP-seq accurately identifies m6A-modified RNA regions by extracting RNA, selectively enriching m6A-modified RNA, constructing libraries, and performing high-throughput …
Magna MeRIP m6A Kit- Transcriptome-wide Profiling of N6 …
The Magna MeRIP m6A Kit streamlines the MeRIP (Methylated RNA Immunoprecipitation) method to enable identification and transcriptome-wide profiling of m6A (N6-methyladenosine) …
RNA N6-methyladenosine modification-based biomarkers for
Oct 30, 2023 · The MeRIP-qPCR results showed that the m 6 A levels of NCOA4 mRNA from monkeys PBMCs increase significantly after irradiation and peak at day 14 post-irradiation …
m6A-dependent glycolysis enhances colorectal cancer progression ...
Apr 3, 2020 · Real-time PCR and MeRIP-qPCR assays were performed to measure the mRNA and m 6 A levels of eighteen hypo-down candidates in different cells. Real-time PCR data …
MeRIP-seq Protocol - CD Genomics
MeRIP-seq (Methyled RNA Immunoprecipitation Sequencing), a high-throughput sequencing technique that combines ChIP-Seq and RNA-Seq methodologies, is utilized for the thorough …
MeRIP Sequencing - CD Genomics
Comprehensive platform: MeRIP experiment—Library construction sequencing analysis—MeRIP qPCR validation, providing truly one-stop service. Flexible selection: Options for library …
MeRIP and m6A-seq Assays - EpigenTek
Techniques such as methylated RNA immunoprecipitation sequencing (MeRIP) (MeRIP-seq, or m6A-seq), as well as analogous methods like PA-m6A-seq, miCLIP, and m6A-CLIP, have …