Structural features and active site analysis of SS isoforms. Maize is shown as an example. (A) Compositions and distributions of domain structures and conserved motifs of SS proteins are marked and annotated in different colours.

6 days ago · Maize ( Zea mays ) is a crop of significant global importance, yet its productivity is considerably hindered by salt stress. In this study, we investigated two maize cultivars, one exhibiting high salt tolerance (ST) and the other showing salt sensitivity (SS) at the seedling stage. The ST cult …

This study identified the complement of soluble starch synthases (SSs) present in developing maize (Zea mays) endosperm. The product of the du1 gene, DU1, was shown to be one of the two major soluble SSs.

Here we use high-density SNP genotyping to characterize a set of North American maize lines spanning the history of modern breeding. We provide a unique analysis of genome-wide developments in genetic diversity, ancestry, and selection.

Apr 15, 2016 · The current study utilized recombinant maize SSI, SSIIa, and SSIII, and fragments of SSIII, to address three aspects of SS function in amylopectin biosynthesis. The first aspect is regulatory interactions between different SS classes.

Aug 24, 2018 · To further explore the more subtle differences among SS isoforms, we aligned the maize SSS protein sequences with the reported Escherichia coli glycogen synthase (EcGS) and barley SSI protein...

Nov 24, 2020 · Immunoblotting of maize amyloplast extracts following non-denaturing polyacrylamide gel electrophoresis identified multiple bands of SSIIa, the electrophoretic mobilities of which were markedly altered by conditions that affected protein phosphorylation, including protein kinase inhibitors.

Nov 18, 2023 · The SS-II enzyme has been found to exist in maize in three different isoforms (SS-IIa, IIb, and IIc); while the SS-IIa and SS-IIc genes have been found to express themselves significantly in the endosperm [29], the SS-IIb gene is mostly expressed in leaves.

Feb 1, 1999 · Maize SSI and maize SSIIb exhibit higher Vmax with glycogen as a primer, while the converse is true for SSIIa. However, the specific activity of SSIIb is at least two- to threefold higher than that for either SSI or SSIIa.

Therefore, in this study, we determined whether maize SS can catalyze the de novo synthesis of α-glucan and studied how maize SS utilizes potential native primers such as maltooligosaccharides (MOS) and developing native starch granules.