In the present study, the effects of the MYC point mutation T58A on the progression of a cellular tumor antigen p53 (p53) −/− human breast cancer cell line was analyzed, and the mechanism of p53-independent MYC-induced apoptosis was investigated. HCC1937 cells were transfected with mutant (T58A) or wild-type (WT) MYC using lentiviral vectors.

Apr 23, 2012 · To examine Myc dependence upon Ras mutations we have generated MMTV regulated Myc and Myc T58A transgenic mice. Expression of the more stable T58A Myc allele resulted in a reduction in...

Sep 7, 2020 · In the T58A mutant, MYC is stabilized with constitutive S62 phosphorylation. When challenged with DMBA/TPA-mediated carcinogenesis, MYC T58A mice had accelerated development of papillomas,...

In this study, Freie et al. demonstrate that a single point mutation (T58A) in MYC's phosphodegron domain stabilizes MYC and deregulates the expression of its target genes, resulting in aberrant self-renewal and apoptotic resistance in hematopoietic progenitors.

Mar 7, 2013 · To examine Myc dependence upon Ras mutations we have generated MMTV regulated Myc and Myc T58A transgenic mice. Expression of the more stable T58A Myc allele resulted in a reduction in KRas-activating mutations.

The c-myc T58A gene bears a single mutation as compared to wild type c-myc and has never been used before to immortalize cells. The T58A mutation has been identified in several cases of Burkitt's lymphomas.

035557 STOCK Igs2 tm1(CAG-Myc*T58A/luc)Wrey /J LSL-Myc T58A mice contain a Cre recombinase regulatable Myc T58A allele under control of a CAG promoter. They are useful as a model of small cell lung cancer (SCLC).

Feb 1, 2011 · Here, we report the development of 3 unique c-myc knock-in mice that conditionally express either c-Myc (WT) or the c-Myc (T58A) or c-Myc (S62A) phosphorylation mutant from the constitutively active ROSA26 locus in response to Cre recombinase to study the role of these phosphorylation sites in vivo.

Feb 14, 2018 · Our results that AZ2 destabilizes the mutant c-Myc (T58A) and accelerates degradation of c-Myc in the presence of the E1 inhibitor (Fig. 3e,f) provide strong evidence that...

Jul 6, 2022 · MYC T58A expressing mice developed clonal T-cell lymphomas with 100% penetrance and conditional knock-out of endogenous Myc accelerated this lymphomagenesis.