Nov 3, 2016 · Here, we identify a molecular mechanism responsible for reciprocal activation between Polo-like kinase-1 (PLK1) and N-Myc. PLK1 specifically binds to the SCF Fbw7 ubiquitin ligase, phosphorylates it, and promotes its autopolyubiquitination and proteasomal degradation, counteracting Fbw7-mediated degradation of N-Myc and additional substrates ...

Dec 3, 2018 · By integrating activity-based proteomic profiling and drug screens, polo-like kinase-1 (PLK1) was identified as an essential regulator of the MYC-dependent kinome in DHL. Notably, PLK1 was expressed at high levels in DHL, correlated with MYC expression, and …

Here, we demonstrate that PLK1 promotes FBXW7 auto poly-ubiquitination and proteasomal degradation, counteracting the FBXW7-mediated degradation of c-MYC in MB cells. In turn, stabilized c-MYC directly activates PLK1 transcription, constituting a regulatory loop.

Mar 18, 2025 · In prostate cancer cell lines, PLK1 promoted the degradation of PDCD4, which released a brake on c-MYC, stimulating its activation of Hedgehog (Hh) signaling and metabolic pathways that reduced cellular dependence on androgen receptor signaling and thus reduced sensitivity to enzalutamide.

We identified a PLK1 selective inhibitor, Volasertib, as the most potent in inhibiting proliferation of glioblastoma cells. PLK1 inhibition induced mitotic catastrophe, G2/M cell cycle arrest, and DNA damage, leading to caspase-mediated apoptosis in glioblastoma cells.

We show that PDK1-PLK1-MYC signaling is critical for cancer cell growth and survival, and small-molecule inhibition of PDK1/PLK1 provides an effective approach for therapeutic targeting of MYC dependency.

identify a molecular mechanism responsible for reciprocal activation between Polo-like kinase-1 (PLK1) and N-Myc. PLK1 specifically binds to the SCFFbw7 ubiquitin ligase, phosphorylates it, and promotes its autopolyubiquitination and protea-somal degradation, counteracting Fbw7-mediated degradation of N-Myc and additional substrates, including ...

May 5, 2013 · In this report, we describe the existence of a MYC-protein kinase A (PKA)-polo-like kinase 1 (PLK1) signaling loop in cells. We report that sequential MYC phosphorylation by PKA and PLK1 protects MYC from proteasome-mediated degradation.

Sep 27, 2017 · Polo-like kinase 1 (PLK1) is a serine/threonine kinase that plays a key role in the regulation of the cell cycle. PLK1 is overexpressed in a variety of human tumors, and its expression level often correlates with increased cellular proliferation and poor prognosis in …

Nov 29, 2018 · Methods and Results: By integrating activity-based proteomic profiling and drug screens in isogenic MYC on/off lymphoma cells, we identified polo-like kinase-1 (PLK1) as an essential regulator of the MYC-dependent kinome in DHL/DEL.