Mesenchymal stem/stromal cells (MSCs) are important players in tissue homeostasis and regeneration owing to their immunomodulatory potential and release of trophic factors that promote healing.

Jul 15, 2022 · Exosomes derived from mesenchymal stem cells (MSCs) have shown to have effective application prospects in the medical field, but exosome yield is very low.

In a transmission electron microscopy (TEM), a thin electron beam transparent specimen (ideally ≤ 100 nm) is exposed to a high-energy (typically 60 - 300 keV) electron beam. Images generally contain contrast that may be due to differences in crystallinity, atomic mass, or thickness variations within the sample.

Mar 24, 2025 · Background Studies examining the therapeutic potential of Mesenchymal stem cells-derived extracellular vesicles (MSC-EVs) in wound healing and skin regeneration have progressed rapidly. Prior to considering clinical translation, a systematic and comprehensive understanding of these experimental details and the overall impact of MSC-EVs on skin regeneration is necessary. Methods 83 studies were ...

Oct 22, 2021 · In vitro results showed that MSC-sEV reduced senescent biomarkers, decreased senescence-associated secretory phenotype (SASP), rescued angiogenesis, migration and other dysfunctions in...

Apr 3, 2017 · MSCs are believed to exert migratory behavior resembling that of leukocytes [8 – 10] with respect to cytokine responsiveness and the ability for transendothelial migration (TEM, diapedesis).

In this paper, we provide a comparative analysis, by TEM, SEM and flow cytometry, of patient colorectal cancer-derived spheroids with that of cancer induced by their mouse xenograft.

MSC-derived exosomes (MSC-exosomes), the main bioactive extracellular vesicles of the paracrine effect of MSCs, have been proposed as a new potential cell-free approach for wound healing...

Mesenchymal stem cell (MSC)-derived exosomes mediate tissue regeneration in a variety of diseases including ischemic heart injury, liver fibrosis, and cerebrovascular disease.

In this protocol, we suggest transmission electron microscopy for the imaging of exosomes including negative staining, whole mount immuno-staining, block preparation, thin section, and immuno-gold labelling.