Performing qRT-PCR assays that can consistently detect BCR-ABL at ≥4.5 log sensitivity (sens) below the standardised baseline is challenging for many labs. Undetectable (undet) BCR-ABL …

Nov 15, 2013 · Based on the validation data, the MRDx BCR-ABL Test is an accurate, reproducible, and highly sensitive IS-standardized solution to the growing need for a reliable and robust quantitative BCR-ABL assay that can be used for the monitoring of minimal residual disease in CML patients. Toplin:MolecularMD: Employment. Drafahl:MolecularMD: Employment.

Dec 6, 2014 · Using the multiplex ddPCR protocol developed 97% of the analysed samples had a sensitivity of MR5.0 or better. Compared to the labs routine qPCR protocol 20% more samples were found positive with ddPCR.

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Dec 6, 2014 · We have tested a multiplex droplet digital PCR (ddPCR) approach in order to obtain reliable measurements at the MR5.0 level. When using a standard laboratory protocol with three reference genes...

ifferent levels of molecular response. MR1: Less than 1 in 10 wh. ood cells (10%) has the BCR-ABL1 gene. MR2: Less than 1 in 100 w. ite blood cells (1%) has the BCR-ABL1 gene. This is the equivalent of complete cytogenetic response on chromosome test. this is what your team might call it. MR3: Less than 1 in 1,000 whi.

Nov 15, 2013 · Real-time quantitative PCR on mRNA BCR-ABL transcripts in addition to total ABL transcripts as internal control has been performed on a LightCycler platform in 1,442 pts within the randomized CML-Study IV and adapted according to the International Scale (IS).

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