MNase-seq is one of four classes of methods used for assessing the status of the epigenome through analysis of chromatin accessibility.

Micrococcal nuclease is derived from Staphylococcus aureus and is a relatively non-specific endo-exonuclease. It is purified from a recombinant E. coli strain that digests double-stranded, …

CUT&RUN sequencing, antibody-targeted controlled cleavage by micrococcal nuclease for transcriptomic profiling. Hydrolysis of nucleic acids in crude cell-free extracts. Sequencing of RNA. Preparation of rabbit reticulocyte lysates. Studies of chromatin structure.

Thermo Scientific Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

May 6, 2016 · We introduce a metric that uses micrococcal nuclease (MNase) digestion in a novel manner to measure chromatin accessibility by combining information from several digests of increasing depths.

MNase is an enzyme that digests DNA in regions that are not stably bound by proteins (Cuatrecasas, Edelhoch, & Anfinsen, 1967). Once the nucleosome is assembled, the DNA wrapped around the histones is protected from MNase digestion, while …

Sep 13, 2019 · Here, we introduce a theoretical framework for predicting nucleosome occupancies and an experimental protocol with appropriate spike-in normalization that confirms our theory and provides accurate occupancy levels over an MNase digestion time course.

Mar 13, 2025 · MNase-Seq, which uses micrococcal nuclease (MNase) digestion followed by sequencing, is widely used to study how DNA is packaged into nucleosomes across the genome. Advancements in this approach have improved resolution, reproducibility, and interpretation of nucleosome patterns.

Jul 8, 2022 · Micrococcal nuclease (MNase) originating from Staphylococcus aureus is a calcium dependent ribo- and desoxyribonuclease which has endo- and exonucleolytic activity of low sequence preference. MNase is widely used to analyze nucleosome positions in chromatin by probing the enzyme’s DNA accessibility in limited digestion reactions.

Here, we report a technique, termed single-cell MNase-seq (scMNase-seq), to measure genome-wide nucleosome positioning and chromatin accessibility simultaneously in single cells.