Treat HEK293T cells with MG132, Time dependant and dose dependant at least 24hrs, time intervals 8hrs. dose you can use 3um, 5um,10um and 15um. It is pretty simple to transfect HEK293 with...

Generally, we are using 40 µM in HEK293T for four hours. Some times we also use 30 µM for 8 hrs in HEK293T to do ubiquitination assay.

(A) HEK293T cells were treated with each 20 µM MG132 or 10 µM lactacystin for the indicated times. Equal amounts of total cell lysates were subjected to Western blot (WB) analysis using...

To address this apparent paradox, quantitative peptidomics was used to study the effect of a variety of other proteasome inhibitors on peptide levels in HEK293T and SH-SY5Y cells. Inhibitors tested included carfilzomib, MG132, MG262, MLN2238, AM114, and clasto-Lactacystin β-lactone.

Jun 23, 2023 · After treatment of 293T cells overexpressing VANGL2 and TBK1 with MG132, 3-MA, or bafilomycin A1 (Baf A1) that blocks autophagosome-lysosome fusion and inhibits acidification and protein degradation in lysosomes, or Caspase inhibitors, Z-VAD-FMK (Z-VAD) and VX-765, we found that the degradation of TBK1 …

Apr 23, 2021 · Only slight changes on cell-cycle progression were observed in MG132-treated 293T cells. The percentage of G1 phase cells was slightly decreased (about 9%), whereas the percentage of G2/M phase cells was slightly increased (about 8%) (Figure S1 B).

HEK 293T cells transfected with empty vector, Myc-ULK1 WT or KI were treated with MG132 and then cell lysates were analysed with indicated antibodies(Top). The ratio of p-p62 and p62 was obtained by dividing the level of p-p62 by total p62(n = 3).

Jan 24, 2013 · Here, we determined the stability and half-life of two ZFN proteins and examined the effect of MG132 (carbobenzoxyl-leucinyl-leucinyl-leucinal-Hl), a proteasome inhibitor, on ZFN-mediated gene modifications. ZFN proteins were expressed in 293T cells after transfection of ZFN-encoding plasmids.

Jul 8, 2019 · Immunoprecipitation of Flag-FBXW7α from HEK-293T cells treated with the proteasomal inhibitor MG132 for 4 h and subsequent mass spectrometry analysis identified several known SCF components...

Apr 1, 2012 · Our data showed that the MG132-induced accumulation of endogenous SUMO-2/3 conjugates was blocked when translation was first abolished by treatment with cycloheximide or puromycin, in all three tested cell lines — HeLa, U2OS and HEK 293T (Fig. 2 A–C, lines 7–10).