002595 C57BL/6-Tg (IghelMD4)4Ccg/J Cells from mice carrying the IghelMD4 transgene recognize hen egg lysozyme and have high serum titers of anti-lysozyme antibodies. This strain may be used to study B-cell selection.

The MD4 line had the highest expression of the transgene and was propogated for further studies. Mice carrying this transgene had high serum titers of anti-lysozyme antibodies. ( J:78308 )

006345 NOD.B6-Tg (IghelMD4)4Ccg/DvsJ These NOD transgenic mice exhibit an increase in the total numbers of splenic B-lymphocytes and delay of diabetes onset in females.

The MD4 mouse strain 23 carries rearranged heavy and light chain transgenes which encode the HEL-specific monoclonal antibody, HyHEL-10, offering a unique opportunity to study B cell somatic hypermutation in germinal centres during the immune response to a …

Oct 8, 2014 · To demonstrate that the generation of BCR retrogenic mice is feasible per se, we chose the well-characterised Hen-Egg-Lysozyme (HEL)-specific BCR, MD4. A MD4 BCR-transgenic line was generated in the 1980s by Goodnow and co-workers and was used as control in our experiments [1].

Splenic B cells in MD4/ML5 Tg mice showed typical phenotypes of anergic B cells, as they substantially downregulated cell-surface IgM and failed to proliferate or upregulate activation marker expression after BCR stimulation (Figures 6B, C).

BrdU + cells were predominantly localized to the immature CD24 hi (HSA hi) compartment in all mice (not depicted), which comprised 20–30% of HEL + B cells in the MD4, MD4 × AL3, and SW HEL mice and 70–80% in the SW HEL × ML5 mice.

Apr 21, 2017 · MD4 Ig-Tg mice have a more highly penetrant transgene, allowing for higher recovery of Ig-Tg B cells (∼ 95% of total B cells), and were used in all other experiments. Donor and recipient mice...

Recipient Ighel Md4 Transgenic Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

Bioz Stars score, Techniques, Protocol Conditions and more for Md4 Mice, supplied by The Jackson Laboratory. Data for Md4 Mice gathered from related PubMed articles.