Reprogramming immunosuppressive myeloid cells by activated …
Jan 8, 2021 · To confirm the role of reprogramming MDSCs by activated T cells in vivo, we tested the effect of PD-1 mAb on the survival and function of tumor-infiltrating myeloid cells in an ICB …
Cell fusion potentiates tumor heterogeneity and reveals …
To generate in vitro–derived hybrids, we engineered two murine cancer cell lines, colon adenocarcinoma (MC38) and melanoma (B16F10), to stably express Cre recombinase and …
Different syngeneic tumors show distinctive intrinsic tumor …
Feb 28, 2022 · We modeled loss of function (LOF) in four common anti-PD-1 antibody-responsive syngeneic tumors, MC38, Hepa1-6, CT-26 and EMT-6, by systematical depleting a series of …
Late-stage MC38 tumours recapitulate features of human …
Apr 20, 2023 · MC38 colon adenocarcinoma is a widely used preclinical model, yet how it recapitulates human colorectal cancer remains poorly defined. This study investigated the …
Cancer cell-derived type I interferons instruct tumor monocyte ...
Dec 6, 2022 · We assessed the capacity of FACS-purified MC38 and LLC Ly6C high Tu.Mons to suppress or stimulate CD8 + T cell proliferation (Figures 1 J, 1K, and S3 S). MC38 Tu.Mons …
FACS analysis of tumor-infiltrating cDCs and macrophages in MC38 …
Accordingly, they significantly increased the ratios of cDCs and T cells in CD45 + cells in the MC38 tumor, except CCL3 (Figures 5 and 7).
323 Immunogenic syngeneic model MC38-OVA for the preclinical …
The MC38-OVA cells were subcutaneously implanted into immunocompetent mice to evaluate the tumorigenicity and in vivo response to anti-PD-1 antibody treatment. Blood was collected 2 …
FACS Analysis of Tumor and Spleen T Cell Populations from MC38 ...
MC38 colon tumor cells (2×106) were implanted subcutaneously into female C57BL/6 mice. At day 7 post implantation, tumor-bearing mice were randomized and dosed with 10 mg/kg of …
FACS Analysis of Tumor and Spleen T Cell Populations from MC38 …
Sep 9, 2016 · FACS Analysis of Tumor and Spleen T Cell Populations from MC38 Tumor-Bearing Mice. MC38 colon tumor cells (2×10 6) were implanted subcutaneously into female C57BL/6 …
Rapid functional impairment of natural killer cells following tumor ...
Jan 24, 2024 · To do this, we FACS-isolated the CD11b+ and CD49a+ NK cells from MC38 tumors and tested their ability to lyse B16-F10 tumor cells in vitro over 4 h (Fig. 3I).