Feb 13, 2023 · The metastatic Mc38 cells made full use of liver vessels and perivascular TAMs; they further adopted VM vessels to match the rapid growth of tumor vasculature.

CD11b + F480 + TAMs were isolated from MC38 (H) and KPC (I) tumours 5 days following irradiation (±aCSF), and expression of selected immune stimulatory and immunosuppressive genes was analysed by RT–qPCR (n = 3).

Feb 13, 2023 · Tumor-associated macrophages (TAMs) are highly heterogeneous and play vital roles in tumor progression. Here we adopted a C57BL/6 mouse model imitating the late-stage colorectal liver metastasis (CRLM) by Mc38 colorectal cancer cell injection via the portal vein.

Here we chose to dissect the function of immunosuppressive Arg1+ TAM in an immunotherapy-responsive MC38 mouse tumor model during aPD-1 treatment. We used the syngeneic MC38 tumor model given its widespread use in immune-oncology and high macrophage content 10.

May 31, 2016 · To investigate whether it was expressed in tumor stroma of mice, we investigated mammary carcinoma (4T1) injected in mammary fat pads as well as in melanoma (B16) and colon carcinoma (MC38) growing subcutaneously (s.c.).

Jun 7, 2024 · Diet-induced obesity (DIO) can alter the tumour immune landscape 24. A syngeneic subcutaneous tumour model using a colorectal adenocarcinoma cell line, MC38, was initially used to test the...

Dec 30, 2019 · We observed that anti-CD40 either alone or in combination with anti-VEGFA/Ang2 markedly reduced the frequency of CD11b + Ly6C − Ly6G – F480 + macrophages (TAMs) in the leukocyte infiltrate of MC38 tumors (Fig. 3 A).

Feb 1, 2023 · F4/80+ TAMs showed proximity to neovascularization and tumor vessels, functionally angiogenic in vivo; and greatly promoted the activation of a few key angiogenic markers such as VEGFA, Ki67,...

Jul 7, 2020 · We identify a proinflammatory F480 + MHCII + Ly6C lo macrophage population that is associated with response rather than resistance to avelumab. These macrophages are the primary source of the interferon-inducible chemokine Cxcl9, which facilitates the recruitment of protective Cxcr3+ T cells.

In our syngeneic mouse CRLM model, survived Mc38 cells in the liver may in time create a tumor microenvironment that favored Kupffer cells to proliferate in situ and/or myeloid monocyte/macrophages to infiltrate, both differentiated into tumor-promoting F4/80 + TAMs.