Feb 28, 2022 · In the first set of experiments, we systematically depleted syngeneic tumor-bearing mice (MC38, Hepa1-6, CT-26, and EMT-6) of different immune-lineages using the following reagents 16, 18: (1)...

Aug 7, 2024 · In the present study, we report a localized cytokine therapy that safely elicits systemic antitumor immunity by targeting the ubiquitous leukocyte receptor CD45.

Mar 30, 2023 · The neutrophils infiltrating MC38 tumors after aCD40 treatment showed the same increase in CD14 and loss of CD101 as in the KP model (Figure 6 A) and highly expressed CXCL10, a marker of the ISG response (Figures 4 H and 6 A).

To focus on cancer cell subpopulations from residual MC38 tumors after treatment, we sorted CD45/CD90 double-negative cells (enriched for tumor cells) and compared them with CD45/CD90 double-positive immune cells (Figure 1F).

Nov 5, 2020 · To focus on cancer cell subpopulations from residual MC38 tumors after treatment, we sorted CD45/CD90 double-negative cells (enriched for tumor cells) and compared them with CD45/CD90 double-positive immune cells (Figure 1F).

We used scRNA seq to characterize the transcriptional profile of CD45+ immune cells in the MC38 tumor model. MC38 tumor digests were FACS sorted for CD45 positivity and >1,000 cells per sample were analyzed by single-cell transcriptomic analysis.

Dec 9, 2020 · Here, we demonstrate that high-fat diet (HFD)-induced obesity impairs CD8 + T cell function in the murine TME, accelerating tumor growth. We generate a single-cell resolution atlas of cellular metabolism in the TME, detailing how it changes with diet-induced obesity.

Jan 8, 2021 · Blockade of the IFN-α/β and TNF-α pathways reduces the efficacy of anti-PD-1 therapy. a, b CD45 + leukocytes from the tumor tissue of MC38 mice were isolated to detect the MDSC number (left ...

We have identified the presence of cancer cells harboring immune cell–specific surface marker proteins such as CD4 in the tumor microenvironment. Cancer cells acquired not only the T cell marker protein CD4 but also immune regulatory molecules such as CTLA4 by trogocytosis.

In the MC38 model, combination treatment significantly promoted CD8 + T cell infiltration and activation in the tumor tissue ( Figure 4 A-D). In addition to T cells, the combination treatment...