Maltose-binding protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes.

Product: maltose binding protein from E. coli This gene encodes a signal sequence that can direct MBP to the bacterial periplasm. ORF: 1 .. 1176 = 1176 bp

We isolated mutations in MBP that enhance binding to maltodextrins 1.3 to 15-fold, using random mutagenesis followed by screening for enhanced yield in a microplate-based affinity purification.

Maltose binding protein (MBP) is a common protein expression tag, as it is known to significantly enhance the solubility of many proteins. MBP is one of the most well-known and accomplished means of tagging proteins expressed in microbes.

The maltose-binding protein (MBP) is one of the constituents of the E. coli maltose/maltodextrin system. Maltose is a preferred nutrient for the E. coli cells, and the maltose/maltodextrin system is a set of proteins including regulators, transporters, and enzymes.

Maltose binding protein (MBP) is used in recombinant protein expression as an affinity and solubility tag. The monoclonal antibody B48 binds MBP tightly and has no cross‐reactivity to other proteins in an Escherichia coli lysate.

Mar 19, 2025 · Maltose-binding protein (MBP) is a transport protein that binds to maltose and maltodextrins. It is not an enzyme, but it was the first protein with which the method of the DNA springs was tried out. MBP is a classic example of induced fit binding, with its general structure consisting of two lobes that tilt towards each other upon binding of ...

In summary, this study establishes MBP as a versatile expression tag for protein production in eukaryotic expression systems. Recombinant expression of extracellular, intracellular as well as transmembrane proteins is essential for biochemical, structural, functional, and …

Jan 1, 2010 · Maltose-binding protein (MBP) is one of the most popular fusion partners being used for producing recombinant proteins in bacterial cells. MBP allows one to use a simple capture affinity step on amylose–agarose columns, resulting in a …

The affinity of maltose-binding protein (MBP) for maltose and related carbohydrates was greatly increased by removal of groups in the interface opposite the ligand binding cleft.