pDB.His.MBP.3C CARD8 CARD WT - Addgene
Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA-CARD
A dual protease approach for expression and affinity …
We describe a new method for affinity purification of recombinant proteins using a dual protease protocol. Escherichia coli maltose binding protein (MBP) is employed as an N-terminal tag to increase the yield and solubility of its fusion …
The use of a commercially available maltose-bind-ing protein (MBP) fusion system (pMAL-c2X; New England Biolabs, Beverly, MA, USA) (3,9) increases sol-ubility. However, Factor Xa …
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NEBExpress® MBP Fusion and Purification System | NEB
The NEBExpress® MBP Fusion and Purification System uses a strong Ptac promoter and the translation initiation signals of MBP to enhance solubility and expression levels of a desired …
HMBP-3C-STAR.66-285 - Addgene
High-yield soluble expression, purification and characterization of human steroidogenic acute regulatory protein (StAR) fused to a cleavable Maltose-Binding Protein (MBP).
Grow cells containing the pMAL-c6T fusion plasmid in LB containing ampicillin and 0.2% glucose to an A600 of ~0.5. Induce by adding IPTG to a final concentration of 0.3 mM. Grow for an …
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Common vectors for protein expression - CSH Protocols
a Unless otherwise specified, all E. coli vectors are IPTG-inducible, have a pBR322-related replicon, and carry a lac repressor gene (lac I or lac Iq). b 6xHis, GST, MBP, Strep (II)-tag, …
MBP Sequence and Map - SnapGene
This gene encodes a signal sequence that can direct MBP to the bacterial periplasm.
MBP fusion protein with a viral protease cleavage site: one-step ...
Affiliation 1 Department of Biochemistry and Biophysics, Box 712, University of Rochester Medical Center, Rochester, NY 14642, USA. [email protected] fusion protein with a …
Purification of MBP fusion proteins using engineered DARPin …
Sep 30, 2021 · Maltose binding protein (MBP) has a long history as an expression tag with the ability to increase the solubility of fused proteins. A critical step for obtaining a sufficient …
