Jan 19, 2023 · In this study, we developed a single-cell m6A sequencing (scm6A-seq) method to simultaneously profile the m6A methylome and transcriptome in single oocytes/blastomeres of …

Here, we describe single-cell DART-seq (scDART-seq), the first method for transcriptome-wide profiling of m 6 A sites in single cells. Using both the droplet-based 10x Genomics and plate …

Feb 17, 2022 · Here, we develop a method to identify m 6 A sites transcriptome-wide in single cells. We uncover surprising heterogeneity in the presence and abundance of m 6 A sites …

Feb 17, 2022 · In this issue of Molecular Cell, Tegowski et al. established a single-cell DART-seq (scDART-seq) method for m6A site profiling and further revealed the fundamental features of …

Sep 7, 2023 · Here, we developed single-nucleus m6A-CUT&Tag (sn-m6A-CT) for simultaneous profiling of m 6 A methylomes and transcriptomes within a single nucleus using mouse …

Aug 16, 2020 · In this system, the targeting element dCas13b is fused to ten copies of GCN4 peptides which can recruit multiple scFv-fusion RNA demethylase to demethylate the target m …

Nov 27, 2013 · Here we show that m6A is selectively recognized by the human YTH domain family 2 (YTHDF2) ‘reader’ protein to regulate mRNA degradation.

In this re-view, we focused on m6A writers and erasers and present an overview on their known functions and enzymatic molecular mechanisms, showing how they recognize substrates and …

Dec 11, 2024 · In this study, we developed an m6A site predictor called deepSRAMP, which integrates sequence-based and genome-derived features into the combination structure of a …

Apr 12, 2022 · He and colleagues have now developed a method, known as m 6 A-selective allyl chemical labelling and sequencing (m 6 A-SAC-seq), that can quantitatively profile …