RNA甲基化免疫共沉淀 （Methylated RNA Immunoprecipitation，MeRIP）基于m6A特异性抗体识别并结合mRNA上的m6A的原理，以 RNA免疫共沉淀 方法富集甲基化修饰片段为基础，并通 …

Mar 12, 2019 · We have used this novel m6A-RT-QPCR approach to quantify the m6A levels in specific residues of human SOCS1 and SOCS3 mRNAs, members of one of the major …

Oct 22, 2018 · Identifying m6A: A radiolabeling-free method enables the rapid detection of N6 -methyladenosine (m 6 A) in rRNA, lncRNA, and mRNA with single-base resolution. It exploits …

Apr 20, 2020 · We found that m 6 A peak overlap in mRNAs varies from ~30 to 60% between studies, even in the same cell type. We then assessed statistical methods to detect changes in …

Mar 12, 2019 · N6-methyladenosine (m6A) is the most common and abundant RNA modification. Recent studies have shown its importance in the regulation of several biological processes, …

Presented here is a protocol for employing meRIP-seq to profile the RNA m 6 A landscape in senescent human cells. We described, in detail, sample preparation, mRNA isolation, …

Sep 20, 2024 · Our findings unveil an m 6 A reading mechanism by CENPA that epigenetically governs centromere integrity in cancer cells, providing potential targets for cancer therapy.

Here, we describe a simple RT-QPCR-based approach for the relative quantification of candidate m 6 A regions that takes advantage of the diminished capacity of BstI enzyme to …

Mar 14, 2022 · We developed m 6 A-selective allyl chemical labeling and sequencing (m 6 A - SAC-seq) 23 as an advanced site-specific sequencing method with broad application …

Here, we describe a simple RT-QPCR-based approach for the relative quantification of candidate m 6 A regions that takes advantage of the diminished capacity of BstI enzyme to …