Similar to chromatin immunoprecipitation (ChIP), RNA immunoprecipitation (RIP) can be used to detect the association of individual proteins with specific nucleic acids such as mRNAs, …

May 18, 2023 · RIP is an antibody-based technique used to map in vivo RNA-protein interactions and RNA modifications such as m6A and ac4C. The RNA binding protein (RBP) of interest is …

Apr 20, 2020 · The most common method to map m6A and to predict changes in m6A between conditions is methylated RNA immunoprecipitation sequencing (MeRIP-seq), through which …

Oct 3, 2022 · Immunoprecipitation assays for N 6-methyladenosine (m6A)-modified RNA include methylated RNA immunoprecipitation with next-generation sequencing (MeRIP-seq) and m6A …

We have optimized the method by using a monoclonal antibody against m6A modifications and magnetic A/G beads. The streamlined protocol is easy to process and can produce results …

Sep 13, 2018 · The refined m6A MeRIP-seq method is suitable for m6A epitranscriptome profiling in a limited amount of patient tumors, setting the ground for unraveling the dynamics of the …

Jun 22, 2023 · Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale m 6 A RNA immunoprecipitation and sequencing (picoMeRIP–seq) for …

We optimized the key parameters of m 6 A MeRIP-seq, including the starting amount of RNA, RNA fragmentation, antibody selection, MeRIP washing/elution conditions, methods for RNA …

Apr 4, 2024 · m6A-RNA immunoprecipitation (RIP) C11 cell line was collected and lysed by lysis buffer for RIP. The cell extract was incubeated with magnetic beads cojugated with anti-m6A …

Sep 15, 2020 · Conjoint analysis of m6A-RIP-seq and RNA-seq data identified m6A-modified mRNA transcripts that were hyper-methylated or hypo-methylated and significantly …