Jan 27, 2023 · We identify exon junction complexes (EJCs) as m 6 A suppressors that protect exon junction–proximal RNA within coding sequences from methylation and regulate mRNA …

Jul 13, 2023 · Here the authors show exon-intron boundary inhibits m6A deposition at ~100 nt region nearby splice site, enabling m6A distribution hallmark, more stable mRNA and flexible protein coding.

May 28, 2021 · N6-methyladenosine (m6A) is a modification that plays pivotal roles in RNA metabolism and function, although its functions in spliceosomal U6 snRNA remain unknown.

Jun 19, 2018 · We find that early co-transcriptional m6A deposition near splice junctions promotes fast splicing, while m6A modifications in introns are associated with long, slowly processed introns and alternative splicing events.

Jan 19, 2023 · We provide evidence that exclusion from splice junctions is mediated by the exon junction complex (EJC), potentially via physical occlusion, and that previously observed associations between exon-intron architecture and mRNA decay are mechanistically mediated via …

Jan 27, 2023 · We identify exon junction complexes (EJCs) as m 6 A suppressors that protect exon junction-proximal RNA within coding sequences from methylation and regulate mRNA stability through m 6 A suppression.

Jul 13, 2023 · By deep learning modeling, we find that exon-intron boundary represses a proportion (12% to 34%) of m 6 A deposition at adjacent exons (~100 nt to splice site). Experiments validate that m 6 A signal increases once the host gene does not undergo pre-mRNA splicing to produce the same mRNA.

Jan 30, 2023 · This new model more accurately captured m 6 A distribution in exon-containing genes than did m 6 Apred-1, confirming the importance of exon–intron architecture in shaping m 6 A...

Jun 29, 2022 · Our findings establish a mechanism by which the memory of nuclear RNA splicing is covalently etched on an mRNA, in the form of m6A, and determines its cytoplasmic stability, with broad implications on the regulation, function, and evolution of both m6A and mRNA stability.

Regional bias of N6-methyladenosine (m6A) mRNA modification avoiding splice site region, calls for an open hypothesis whether exon-intron boundary could affect m6A deposition.