To accurately characterize liposomes, cryo-Electron Microscopy (cryo-EM) has been employed as it is the most precise and direct method to determine liposome lamellarity, size, shape and ultrastructure.

Aug 4, 2020 · Toward this goal, here we present a convenient workflow for cryo-EM structural analysis of MPs embedded in liposomes, using the well-characterized AcrB as a prototype. Combining optimized proteoliposome isolation, cryo-sample preparation on graphene grids, and an efficient particle selection strategy, the three-dimensional (3D) reconstruction ...

Aug 29, 2023 · Here we use DNA nanostructures to coat, cluster, and pattern sub-100-nm liposomes, generating distance-controlled vesicle networks, strings and dimers, among other configurations. The DNA coating...

Results from negative staining of liposomes should be interpreted carefully and especially unexpected findings should be crosschecked with other methods, for example cryo-EM. Optimization of staining protocols are often needed to adapt to different liposome preparations.

To accurately characterize liposomes, cryo-Electron Microscopy (cryo-EM) has been employed as it is the most precise and direct method to determine liposome lamellarity, size, shape and ultrastructure.

Here, we propose a liposome signal subtraction method based on single-particle two-dimensional (2D) classification average images, aimed at enhancing the reconstruction resolution of membrane proteins.

Jun 1, 2020 · To apply cryo-EM for structural analysis of MPs embedded or attached to liposomes, it is necessary to develop a highly repro-ducible and convenient workflow for membrane protein incorpo-ration, cryo-sample preparation, and cryo-EM data processing. For this purpose, we chose the well-studied multidrug-resistant trans-

Dec 6, 2018 · Here, we present direct, label-free characterization of liposome structure and composition at the site of its interaction with citrate-stabilized gold nanoparticles by surface-enhanced Raman scattering (SERS) and cryogenic electron microscopy (cryo-EM).

Mar 24, 2025 · Detergents and lipid nanodiscs affect the cryo-EM structures of pentameric ligand-gated ion channels (pLGICs) including ELIC. To determine the structure of a pLGIC in a membrane environment that supports ion channel function, we performed single particle cryo-EM of ELIC in liposomes.

Starting from high-resolution analyses of transient receptor potential (TRP) channels (Liao et al. 2013; Paulsen et al. 2015) and γ-secretase (Bai et al. 2015; Lu et al. 2014), structures of various membrane proteins have been revealed by cryo-EM studies.