A Quick Method to Synthesize Extrachromosomal Circular DNA In …
In this study, we introduce a novel method named QuickLAMA (Ligase-Assisted Minicircle Accumulation) for rapidly synthesizing eccDNAs up to 2.6 kb using a simple PCR and ligation …
Circle-seq based method for eccDNA synthesis and its application …
Dec 1, 2024 · The critical step for successful LAMA synthesis involves the preparation of two required dsDNA fragments (linear A and B). Circle-seq is a widely employed method for …
Small extrachromosomal circular DNAs, microDNA, produce short ...
May 5, 2019 · We created synthetic microDNA mimicking known microDNA sequences by utilizing a technique, called ligase-assisted mini-circle accumulation (LAMA), which relies on cycles of …
Unveiling the mysteries of extrachromosomal circular DNA: from ...
Dec 20, 2024 · Extrachromosomal circular DNAs (eccDNAs) are a type of circular DNAs originating from but independent of chromosomal DNAs. Nowadays, with the rapid …
Integrating Circle-Seq with transcriptomics reveals genome-wide ...
Small circular DNA elicited a stronger cytokine response than linear DNA with the same sequence. Our work provided a detailed profiling of eccDNAs in both healthy and DCM hearts …
Genome-wide characterization of extrachromosomal circular DNA …
Nov 26, 2024 · Extrachromosomal circular DNAs (eccDNAs) are defined as distinct genomic entities of circular and mobile DNA molecules, but their molecular functions in and impact on …
(A) Diagram of artificial microDNA creation by LAMA. (B) Circular …
... created synthetic microDNA mimicking known microDNA sequences by utilizing a technique, called ligaseassisted mini-circle accumulation (LAMA), which relies on cycles of annealing,...
Distribution and characterization of extrachromosomal circular DNA …
Dec 2, 2022 · Here, we synthesized eccDNA by LAMA to preliminarily verify the function of eccDNAs in different gene regions. Therefore, this paper helps bridge the gap in the existing …
Extrachromosomal circular DNA in cancer: history, current …
Jul 1, 2022 · Extrachromosomal circular DNA (eccDNA) is a closed-circle, nuclear, nonplasmid DNA molecule found in all tested eukaryotes. eccDNA plays important roles in cancer …
Preparation of DNA minicircles from linear duplexes with very long ...
A single-stranded circular 80-mer DNA with an A 11 region was synthesized using a ligase-assisted minicircle accumulation (LAMA) protocol (38). The A 11 strand (40 pmol) was …