Apr 1, 2020 · Here we show that the low-density lipoprotein receptor-related protein 1 (LRP1) controls the endocytosis of tau and its subsequent spread. Knockdown of LRP1 significantly reduced tau...

Sep 6, 2022 · The decreased chondrogenesis caused by LRP1 deficiency was rescued by the treatment of PNU-74654 (PNU; a β-catenin antagonist) in ATDC5 cells transfected with short hairpin RNA (shRNA)-Lrp1, which illustrated that β-catenin inhibition could be a therapeutic target for decreased chondrogenic ability caused by LRP1 deficiency.

In this work, we demonstrate that LRP1 inhibits apoptosis in primary neurons under stress conditions by regulating insulin receptor and Akt signaling pathway. LRP1 shRNA plasmids TRCN119624 (target 1) and TRCN119625 (target 2) and the …

We injected adeno-associated virus AAV9-SP-A-LRP1 shRNA into the tail vein of DM mice to selectively knock down LRP1 gene expression in cerebral vascular endothelial cells. Then, the cognitive function and the expression levels of Aβ and Aβ clearance-related proteins in the brains of normal, DM and LRP1 knockdown mice were detected.

These results demonstrate that endogenous LRP1 levels can be efficiently knocked down in mouse primary neurons by lentivirus-delivered shRNA. Next, we determined the effects of LRP1 knockdown...

Compared with control shRNA treatment, we found that LRP1 knockdown (KD) significantly reduced IRβ levels in GT1–7 cells in both the presence and absence of insulin (Fig. 1A,B). Whereas insulin stimulated phosphorylation of Akt, the levels of phosphorated Akt (p-Akt) were significantly lower in LRP1-KD cells ( Fig. 1 A , B ).

Dec 12, 2014 · LRP1 regulates GluA1-mediated neurite outgrowth and filopodia formation. Mouse primary neurons were infected with lentivirus carrying control vector or GluA1 cDNA and lentivirus carrying NT-shRNA or LRP1-shRNA.

Jul 29, 2010 · We decreased the expression of clathrin heavy chain by lentiviral-mediated delivery of small hairpin interfering RNA (shRNA) in N2a-mLRP4 cells and analyzed cell surface levels of mLRP4 by FACS.

May 14, 2021 · Here we show that the low-density lipoprotein (LDL) receptor-related protein 1 (LRP1) controls tau endocytosis and subsequent spread. Knockdown of LRP1 significantly reduced tau uptake in H4 neuroglioma cells and iPS-derived neurons.

Dec 9, 2024 · We found that the PCSK9 rs562556 variant drives breast cancer metastasis by targeting tumoral low-density lipoprotein (LDL) receptor (LDLR) related protein 1 (LRP1) receptors, consequently leading to the induction of genes that we implicate as metastatic colonization promoters.