LPS performs several functions in Gram-negative bacteria. The most fundamental function of LPS is to serve as a major structural component of the OM. Perhaps not surprisingly, LPS is an essential component of the cell envelope in most, although …

To alter properties of the outer membrane or evade the host immune response, Gram-negative bacteria employ a wide variety of chemical modifications to alter LPS. Here we review key features and physiological consequences of LPS biogenesis and modifications.

Apr 17, 2023 · Lipopolysaccharides (LPS) are important outer membrane components of gram-negative bacteria. They are large amphipathic glycoconjugates that typically consist of a lipid domain (hydrophobic) attached to a core oligosaccharide and a distal polysaccharide. These molecules are also known as lipoglycans due to the presence of lipid and sugar molecules.

In most Gram-negative bacteria, the OM is an asymmetric bilayer of phospholipid and lipopolysaccharides (LPS), the latter exclusively found in the outer leaflet. A typical LPS molecule consists of three parts ( Figure 1 ): 1) lipid A, a glucosamine-based phospholipid, 2) a relatively short core oligosaccharide, and 3) a distal polysaccharide (O ...

Nov 1, 2017 · LPS biogenesis is an excellent target for development of novel antibiotic therapies. The outer membrane (OM) of Gram-negative bacteria is an asymmetric lipid bilayer containing a unique glycolipid, lipopolysaccharide (LPS) in its outer leaflet.

Mar 1, 2022 · LPS assemblies reconstituted at interfaces represent a versatile tool to study the properties of the Gram-negative OM by exploiting several surface-sensitive techniques, in particular X-ray and neutron scattering, which can probe the structure of thin films with sub-nanometer resolution.

May 28, 2024 · The outer membrane (OM) of Gram-negative bacteria acts as an effective barrier to protect against toxic compounds. By nature, the OM is asymmetric with the highly packed lipopolysaccharide (LPS) at the outer leaflet and glycerophospholipids at the inner leaflet.

Mar 30, 2016 · The biogenesis of the functional lipopolysaccharide (LPS) transport (Lpt) outer membrane (OM) translocon, LptDE, requires disulfide bond rearrangements at the OM. LptD and LptE are targeted to...

Nov 1, 2017 · Seven lipopolysaccharide transport proteins, LptA-G, form a trans‑envelope complex that is responsible for LPS extraction from the IM and transporting it across the periplasm to the OM. The LptD/E of the complex transports LPS across the OM and inserts it into the outer leaflet of the OM.

Sep 15, 2023 · Here, we demonstrate that the global reduction in OMPs caused by BamD loss weakens the OM, altering cell shape and causing OM rupture in spent medium. To fill the void created by OMP loss,...