Jan 15, 2018 · In summary, we have built a grating-coupled SPR smartphone spectrometer and applied it for the detection of lipopolysaccharides (LPS). This optical instrument does not contain electronic components and solely relies on the LED flash light source and the CMOS camera built in the used smartphone.

等温滴定量热法 (ITC) 结果显示：SPR-1 与 MD2 的亲和力Kd值为18.8 μM (图2A)， 脂多糖 (LPS) 与 MD2 蛋白的亲和力Kd值为 2.75 μM (图2B)。 表明 SPR-1 发挥免疫调节作用的潜在机制是通过与 MD2 直接相互作用，激活 MD2/TLR4 信号传导。

The lipopolysaccharide (LPS)-binding protein (LBP) has a concentration-dependent dual role in the pathogenesis of gram-negative sepsis: low concentrations of LBP enhance the LPS-induced activation of mononuclear cells (MNC), whereas the acute-phase ...

Jun 24, 2020 · Our findings indicate that in human epithelial cells GBP1 acts as a cytosolic LPS sensor and assembles a platform for caspase-4 recruitment and activation at LPS-containing membranes as the...

Jun 3, 2024 · Here, we explore the binding mechanism of polymyxin B to bacterial cells, outer membrane vesicles (OMVs), and pure LPS using a variety of SPR-based assay formats augmented with microbiology ...

Aug 6, 2014 · Caspase-11 activation-induced pyroptosis, in response to cytoplasmic lipopolysaccharide (LPS), is critical for endotoxic shock in mice. The mechanism underlying cytosolic LPS sensing and...

SPR analysis at low pH of 6.5 and 5.5 was performed to investigate the importance of electrostatic interactions in binding of the peptides to LPS. At a pH of 5.5, peptide B59 displayed 10× stronger affinity for lipid A (supplemental Fig. S4 A).

Dec 20, 2012 · The binding affinity and structure activity relationships (SAR) of the AGP-LPS interactions were characterized by surface plasma resonance (SPR).

Apr 1, 2008 · LPS-binding protein (LBP) is a central mediator that transfers LPS to CD14 to initiate TLR4-mediated proinflammatory response. However, a possibility of another LPS transfer molecule has been suggested because LBP-deficient mice showed almost normal inflammatory response after LPS injection.

Sep 30, 2022 · Due to the ability of surface plasmon resonance (SPR) biosensor to confirm the specific interactions between receptors and ligands, a new configuration of SPR biosensor proposed to detect the possible interaction between 10-HDA and TLR4.